Lymphotoxin alpha and Lymphotoxin beta are pro-inflammatory TNF superfamily ligands that play important roles in immune system development. LT-alpha can be secreted as a homotrimer that binds and activates TNF RI, TNF RII, HVEM, and TROY. It is required for development of the spleen, lymph nodes, and Peyer’s patches. It also regulates T cell homing to the gut and IgA induction. LT-alpha can additionally form membrane-associated heterotrimers with two copies of LT-beta/TNF-beta on the surface of B, T, LTi, and ILC3 cells. This heterotrimer binds and activates the Lymphotoxin beta R on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV). The heterotrimer promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by IEC.
Lymphotoxin Antibody (135125) [Dylight 405]
Novus Biologicals | Catalog # FAB1370E
Key Product Details
Species Reactivity
Human
Applications
Neutralization
Label
DyLight 405 (Excitation = 400 nm, Emission = 420 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 135125
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Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Lymphotoxin alpha / beta
Specificity
Recognizes human Lymphotoxin alpha chains in alpha 2 beta 1 or alpha 1 beta 2 trimers and also human TNF-beta. In direct ELISAs, less than 10% cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhEDA-A2, rhFas Ligand, rhLIGHT, rhGITR Ligand, rhOX40 Ligand, recombinant mouse RANKL, rhTNF-alpha, rhTRAIL, rhTRANCE, rhTWEAK, or rhVEGI is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications for Lymphotoxin Antibody (135125) [Dylight 405]
Application
Recommended Usage
Neutralization
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Formulation
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C in the dark.
Background: Lymphotoxin
Alternate Names
LT, Lymphotoxin alpha, tnfb, TNFSF1, TNLG1E
Gene Symbol
LTA
Additional Lymphotoxin Products
Product Documents for Lymphotoxin Antibody (135125) [Dylight 405]
Product Specific Notices for Lymphotoxin Antibody (135125) [Dylight 405]
DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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