Human Mena Alexa Fluor® 405-conjugated Antibody

Catalog #: AF5304V Datasheet / COA / SDS

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AF5304V-100UG

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Human Mena Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Mena in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Mena
Asn386-Arg513
Accession # Q8N8S7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Mena

Mena (mammalian ENA; also protein enabled homolog) is a cytosolic member of the Ena/VASP family of proteins. It runs anomalously high in SDS-PAGE due to a high proline content. Multiple cell types produce Mena, including fibroblasts, myoepithelial cells, neurons and endothelium. Mena promotes the organization of actin filaments in filopodia, and strengthens cell-cell interactions in epithelia. Human Mena is 591 amino acids (aa) in length. It contains one WH1 domain (aa 1‑111), two coiled-coil regions (aa 135‑265 and 557‑587), a Pro-rich segment (aa 310‑373) and an EVH domain (aa 391‑588) that binds G- and F-actin, and mediates tetramerization. There is a 140 kDa neuronal isoform that shows a large 228 aa insertion after Ala268.

Entrez Gene IDs

55740 (Human); 360891 (Rat)

Alternate Names

ENA; enabled homolog (Drosophila); ENAH; FLJ10773; Mena; MENANDPP1; NDPP1; protein enabled homolog