Human Mena Alexa Fluor® 700-conjugated Antibody

Catalog #: AF5304N Datasheet / COA / SDS

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AF5304N-100UG

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Human Mena Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Mena in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Mena
Asn386-Arg513
Accession # Q8N8S7

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Mena

Mena (mammalian ENA; also protein enabled homolog) is a cytosolic member of the Ena/VASP family of proteins. It runs anomalously high in SDS-PAGE due to a high proline content. Multiple cell types produce Mena, including fibroblasts, myoepithelial cells, neurons and endothelium. Mena promotes the organization of actin filaments in filopodia, and strengthens cell-cell interactions in epithelia. Human Mena is 591 amino acids (aa) in length. It contains one WH1 domain (aa 1‑111), two coiled-coil regions (aa 135‑265 and 557‑587), a Pro-rich segment (aa 310‑373) and an EVH domain (aa 391‑588) that binds G- and F-actin, and mediates tetramerization. There is a 140 kDa neuronal isoform that shows a large 228 aa insertion after Ala268.

Entrez Gene IDs

55740 (Human); 360891 (Rat)

Alternate Names

ENA; enabled homolog (Drosophila); ENAH; FLJ10773; Mena; MENANDPP1; NDPP1; protein enabled homolog