Human Methionine Aminopeptidase 1D/MAP1D Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4714S Datasheet / COA / SDS

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AF4714S-100UG

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All Methionine Aminopeptidase 1D/MAP1D Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Methionine Aminopeptidase 1D/MAP1D Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human Methionine Aminopeptidase 1D/MAP1D in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Methionine Aminopeptidase 1D/MAP1D
Arg44-Ala335
Accession # Q6UB28

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Methionine Aminopeptidase 1D/MAP1D

Methionine Aminopeptidase 1D (MAP1D) is a member of the Methionine Aminopeptidase family (MetAPs) (1). MetAPs catalyze the removal of the initiator methionine residue from nascent peptides (2) and are essential for cell growth (3). MAP1D is localized in mitochondria (4) and is over-expressed in colon cancer cell lines and colon tumors (1). The N-terminal leader peptide (residue 1‑43) of MAP1D has been shown to be responsible for targeting to mitochondria (4).

Entrez Gene IDs

254042 (Human)

Alternate Names

EC 3.4.11.18; MAP1D; MAP1DCDS of metAP-3 within PCR fragment; MetAP1D; Metap1l; Methionine Aminopeptidase 1D; methionine aminopeptidase 1D, mitochondrial; methionyl aminopeptidase type 1D (mitochondrial); Methionyl aminopeptidase type 1D, mitochondrial