Human/Mouse BID Alexa Fluor® 647-conjugated Antibody

Catalog #: AF860R Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF860R-100UG

Save 15% on Select RUO Reagents. See Details.

All BID Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse BID Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse BID, a fragment generated in cells treated with anti-Fas and the carboxyl‑terminal portion of recombinant BID generated by cleavage with Caspase 8 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse BID
Met1-Asp195
Accession # AAC71064

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BID

BID is a 195 amino acid member of the Bcl-2 family of proteins that regulates outer mitochondrial membrane permeability (1). BID is a pro-apoptotic member that causes cytochrome c to be released from the mitochondria intermembrane space into the cytosol. In healthy cells BID is cytosolic. In response to Fas ligand or TNF, BID is cleaved by caspase-8 and it then relocates to the mitochondria outer membrane (2, 3). Cleavage of BID by caspase-8 generates a new N-terminal that contains a terminal glycine. It appears that the glycine is myristoylated and myristoylation serves to target BID to the mitochondria (4). BID may then interact with another pro-apoptotic Bcl-2 family member Bak (5). Interaction of BID with Bak causes altered mitochondrial membrane permeability. A 9‑13 amino acid stretch called the BH3 region (Bcl-2 homology region) appears to mediate the BID interaction with other Bcl-2 family members. BID is neutralized by binding to the anti-apoptotic member Bcl-xL.

Long Name

BH3 Interacting Domain Death Agonist

Entrez Gene IDs

637 (Human); 12122 (Mouse)

Alternate Names

apoptic death agonist; BH3 interacting domain death agonist; BH3-interacting domain death agonist; BID isoform ES(1b); BID isoform L(2); BID isoform Si6; BID; desmocollin type 4; FP497; Human BID coding sequence; MGC15319; MGC42355; p22 BID; tbid