Human/Mouse BMP-8b Alexa Fluor® 488-conjugated Antibody

Catalog #: AF6305G Datasheet / COA / SDS

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AF6305G-100UG

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Human/Mouse BMP-8b Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse BMP-8b in direct ELISAs and human and mouse in Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse (rm) BMP-8a and less than 1% cross‑reactivity with recombinant human (rh) BMP‑3b, r

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse BMP-8b
Thr261-His399
Accession # NP_031585

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: BMP-8b

BMP-8b (Bone morphogenetic protein 8b; also OP-3) is a 16 kDa predicted mature and 45 kDa precurser member of the Gbb-60A class of the BMP family of molecules. In mouse, precursors to sperm and oocytes arise from the proximal epiblast around day E7.5 (days 18-20 in human). This lineage commitment is a response to BMP-4 signaling, which is promoted by the repressive action of BMP-8b on the anterior visceral endoderm, a source of BMP-4 antagonism. Mouse BMP-8b preproprecursor is 399 amino acids (aa) in length. It contains a 19 aa signal sequence, a 241 aa prosegment, and a 139 aa mature region. An interchain disulfide bond occurs at Cys363, generating a homodimer. Over aa 261-399, mouse BMP-8b shares 74% aa identity with mouse BMP-8a, and 69% and 93% aa identity with human and rat BMP-8b, respectively.

Long Name

Bone Morphogenetic Protein 8b

Entrez Gene IDs

656 (Human); 12164 (Mouse)

Alternate Names

BMP-8; BMP-8b; bone morphogenetic protein 8b; MGC131757; OP-3