Human/Mouse Bub3 Alexa Fluor® 405-conjugated Antibody

Catalog #: AF5706V Datasheet / COA / SDS

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AF5706V-100UG

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Human/Mouse Bub3 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse Bub3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human Bub1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Bub3
Met1-Thr328
Accession # O43684

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Bub3

Bub3 (Budding uninhibited by benzimidazoles 3 homolog; also Mitotic checkpoint protein Bub3) is a 35‑40 kDa member of the WD repeat Bub3 family of molecules. It plays a key role in chromosome segregation by serving as a component of the mitotic checkpoint complex. This complex inhibits Cdc20, and E3 ubiquitin ligase that initiates the metaphase to anaphase transition, until microtubules are properly aligned with, and bound to, chromatid kinetochores. Human Bub3 is 328 amino acids (aa) in length. It contains five WD40 repeats (aa 5‑43, 46‑83, 86‑124, 128‑163 and 223‑262) plus a phosphorylation site at Ser211 and a Lys at # 216 that may form an interchain crosslink with Gly. There are two splice variants that show two or four aa deletion over the four C‑terminal amino acids. Full‑length human and mouse Bub3 share 99% aa identity.

Long Name

BUB3 Budding Uninhibited By Benzimidazoles 1 Homolog

Entrez Gene IDs

9184 (Human); 12237 (Mouse)

Alternate Names

BUB3 (budding uninhibited by benzimidazoles 3, yeast) homolog; BUB3 budding uninhibited by benzimidazoles 3 homolog; Bub3; BUB3L; budding uninhibited by benomyl; budding uninhibited by benzimidazoles 3 homolog (yeast); hBUB3; mitotic checkpoint component; mitotic checkpoint protein BUB3