Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse

Applications

Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CHD1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CHD1
Lys1531-Thr1710
Accession # O14646

Specificity

Detects human and mouse CHD1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human CHD5 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse CHD1 Antibody

Detection of Human CHD1 antibody by Western Blot.

Detection of Human CHD1 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse CHD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6195) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD1 at approximately 210 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Western Blot Shows Human CHD1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human CHD1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HEK293T human embryonic kidney parental cell line and CHD1 knockout HEK293T cell line (KO). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse CHD1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6195) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CHD1 at approximately 210 kDa (as indicated) in the parental HEK293T cell line, but is not detectable in knockout HEK293T cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse CHD1 Antibody

Application
Recommended Usage

Knockout Validated

CHD1 is specifically detected in HEK293T human embryonic kidney parental cell line but is not detectable in CHD1 knockout HEK293T cell line.

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CHD1

CHD1 (Chromohelicase/ATPase DNA‑binding protein 1) is a 200‑220 kDa member of the SNF2/RAD54 helicase family of proteins. It is an ATP‑dependent chromatin remodeling factor that helps maintain chromatin in a transcriptionally active state. In embryonic stem cells, CHD1 associates with the promoters of active genes, a condition that is associated with open chromatin and pluripotency. Human CHD1 is 1710 amino acids (aa) in length. It contains two Ser‑rich regions (aa 1‑70 and 117‑137), two Chromo (chromatin‑organizer‑modifer) domains (aa 272‑364 and 389‑452), a SNF2 family N‑terminal domain (aa 484‑763) and a C‑terminal helicase domain (aa 792‑943). There are at least 30 Ser/Thr phosphorylation sites. Over aa 1531‑1710, human CHD1 shares 92% aa identity with mouse CHD1.

Long Name

Chromodomain Helicase DNA Binding Protein 1

Alternate Names

ATP-dependent helicase CHD1, CHD-1, chromodomain helicase DNA binding protein 1, chromodomain-helicase-DNA-binding protein 1, DKFZp686E2337, EC 3.6.1, EC 3.6.4.12

Entrez Gene IDs

1105 (Human); 12648 (Mouse)

Gene Symbol

CHD1

UniProt

O14646 (Human)

Additional CHD1 Products

Product Documents for Human/Mouse CHD1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse CHD1 Antibody

For research use only

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