Human/Mouse DAPP1 Alexa Fluor® 405-conjugated Antibody

Catalog #: FAB7024V Datasheet / COA / SDS

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FAB7024V-100UG

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Human/Mouse DAPP1 Alexa Fluor® 405-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human DAPP1 in direct ELISAs. Detects human and mouse DAPP1 in Western blots.

Source

Monoclonal Mouse IgG2a Clone # 716804

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human DAPP1
Gly2-Ser163
Accession # Q9UN19

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 405 (Excitation= 405 nm, Emission= 421 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DAPP1

DAPP1, also known as Bam32, is a 32 kDa intracellular adaptor protein for tyrosine-phosphorylated proteins and 3-phosphoinositides. It is expressed in B cells, T cells, and mast cells and plays a role in signal transduction during the activation of these cell types. DAPP1 is also required for progression of germinal center reactions and the adhesion of B cells to T cells. It is recruited to the plasma membrane following PI 3-kinase activation. DAPP1 contains one SH2 (aa 34-129) and one PH (aa 164-259) domain. Within aa 2-163, human DAPP1 shares approximately 90% aa sequence identity with mouse and rat DAPP1. Alternate splicing generates an additional isoform with a 22 aa substitution at the C-terminus.

Long Name

Dual Adaptor of Phosphotyrosine and 3-Phosphoinositides 1

Entrez Gene IDs

27071 (Human); 26377 (Mouse); 362046 (Rat)

Alternate Names

B lymphocyte adapter protein Bam32; BAM32; BAM32DKFZp667E0716; B-cell adapter molecule of 32 kDa; DAPP1; dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide; dual adaptor of phosphotyrosine and 3-phosphoinositides; hDAPP1