Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 716804
View all DAPP1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DAPP1
Gly2-Ser163
Accession # Q9UN19

Specificity

Detects human DAPP1 in direct ELISAs. Detects human and mouse DAPP1 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse DAPP1 Antibody

Detection of Human and Mouse DAPP1 antibody by Western Blot.

Detection of Human and Mouse DAPP1 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse DAPP1 Monoclonal Antibody (Catalog # MAB7024) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DAPP1 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse DAPP1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, Daudi human Burkitt's lymphoma cell line, Ramos human Burkitt's lymphoma cell line, and BaF3 mouse pro-B cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DAPP1

DAPP1, also known as Bam32, is a 32 kDa intracellular adaptor protein for tyrosine-phosphorylated proteins and 3-phosphoinositides. It is expressed in B cells, T cells, and mast cells and plays a role in signal transduction during the activation of these cell types. DAPP1 is also required for progression of germinal center reactions and the adhesion of B cells to T cells. It is recruited to the plasma membrane following PI 3-kinase activation. DAPP1 contains one SH2 (aa 34-129) and one PH (aa 164-259) domain. Within aa 2-163, human DAPP1 shares approximately 90% aa sequence identity with mouse and rat DAPP1. Alternate splicing generates an additional isoform with a 22 aa substitution at the C-terminus.

Long Name

Dual Adaptor of Phosphotyrosine and 3-Phosphoinositides 1

Alternate Names

BAM32

Entrez Gene IDs

27071 (Human); 26377 (Mouse); 362046 (Rat)

Gene Symbol

DAPP1

UniProt

Q9UN19

Additional DAPP1 Products

Product Documents for Human/Mouse DAPP1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse DAPP1 Antibody

For research use only

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