Human/Mouse EBF-1 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF5165T Datasheet / COA / SDS

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AF5165T-100UG

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All EBF-1 Antibodies

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Human/Mouse EBF-1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse EBF-1 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant mouse EBF-1
Arg416-Ser520
Accession # Q07802

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: EBF-1

EBF-1 (Early B cell Factor 1; also OLF1 and COE1) is a 65-70 kDa member of the COE family of transcription factors. Although expressed in adipocytes and neurons, it is best studied in B cells where IL-7 acts to promote EBF-1 in pre-proB cells, leading to proB stage development. Mouse EBF-1 is 591 amino acids (aa) in length. It contains one DNA-binding region with an embedded zinc-finger motif (aa 51‑235), a dimerization segment between aa 370‑430, and a Pro/Ser-rich transactivation domain (aa 462‑550). EBF-1 either homodimerizes, or heterodimerizes with EBF-2 and -3. There is an alternate start site at Met134, and an isoform that shows a one aa substitution for aa 252‑259. Over aa 416‑520, mouse EBF-1 shows absolute aa identity to the equivalent sequence in rat and human EBF-1.

Long Name

Early B-cell Factor 1

Entrez Gene IDs

1879 (Human); 13591 (Mouse); 116543 (Rat)

Alternate Names

COE1; Collier, Olf and EBF transcription factor 1; early B-cell factor 1; Early B-cell factor; EBF1; EBF-1; EBFFLJ41763; O/E-1; OE-1; OLF1; OLF1FLJ39389; olfactory neuronal transcription factor 1; transcription factor COE1