Human/Mouse Hck Alexa Fluor® 350-conjugated Antibody

Catalog #: FAB3915U Datasheet / COA / SDS

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FAB3915U-100UG

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Human/Mouse Hck Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects endogenous human and mouse Hck at 59 kDa in Western blots.

Source

Monoclonal Rat IgG2a Clone # 394903

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Hck
Met22-Asp80
Accession # P08631

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Hck

Hemopoietic cell kinase (Hck) is a member of the Src family of tyrosine kinases. Hck expression is prominent in cells of myeloid lineage, particularly in granulocytes and monocytes, with protein levels increasing during terminal differentiation and macrophage activation. Hck contains a unique amino-terminal domain that is subject to lipid modifications, targeting this kinase to the plasma membrane.

Long Name

Hemopoietic Cell Kinase

Entrez Gene IDs

3055 (Human); 15162 (Mouse); 25734 (Rat)

Alternate Names

Bmk; EC 2.7.10; EC 2.7.10.2; Hck; Hctk; hemopoietic cell kinaseJTK9; JTK9; p59-HCK/p60-HCK; tyrosine-protein kinase HCK