HO-1/HMOX1/HSP32 Antibody

R&D Systems | Catalog # AF3776

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Knockout Validated, Western Blot, Simple Western

Cited:

Immunohistochemistry, Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HO-1/HMOX1/HSP32 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HO‑1/HMOX1/HSP32
Met1-Thr261
Accession # P09601

Specificity

Detects human and mouse HO‑1/HMOX1/HSP32 in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human HO-2 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HO-1/HMOX1/HSP32 Antibody

Detection of Human/Mouse HO-1/HMOX1/ HSP32 antibody by Western Blot.

Detection of Human/Mouse HO‑1/HMOX1/ HSP32 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). For additional reference, recombinant human HO-1 and HO-2 (5 ng/lane) were included. A specific band for HO-1/HMOX1/HSP32 was detected at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human and Mouse HO‑1/HMOX1/HSP32 antibody by Simple WesternTM.

Detection of Human and Mouse HO‑1/HMOX1/HSP32 by Simple WesternTM.

Simple Western lane view shows lysates of A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-1/HMOX1/HSP32 at approximately 37 kDa (as indicated) using 25 µg/mL of Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Western Blot Shows Human HO-1/HMOX1/HSP32 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human HO‑1/HMOX1/HSP32 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and HO-1/HMOX1/HSP32 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse HO-1/HMOX1/HSP32 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3776) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for HO-1/HMOX1/HSP32 at approximately 32 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of HO-1/HMOX1 by Western Blot

Detection of HO-1/HMOX1 by Western Blot

Generation of CRISPR against INSR gene in human hepatic stellate cells and validation. (A) Diagram of the CRISPR knockout of INSR gene. (B) Real‐time PCR results show the validation of the INSR gene KO on INSR mRNA alternative splicing (*p <.05; **p <.01; ***p <.001; n = 3 each; unpaired T‐test; ±SEM), and (C) Western Blotting showing the protein expression of the insulin receptor (INSR) and heat shock protein 90 (HSP90) as a control in Scramble and INSRe5‐8 KO HSCs (*p <.05; **p <.01; ***p <.001; n = 3 each; unpaired T‐test; ±SEM). (D) BrdU incorporation in the scramble and INSRe5‐8 KO HSCs. (E) Cell growth assay results of scramble and INSRe5‐8 KO HSCs treated with tumor growth factor‐ beta (TGF beta ) 5 ng/mL for 0 to 96 h. *p <.05 in INSR KO Veh v.s. Scramble Veh; $p <.05 in INSR KO TGFb v.s. Scramble TGFb; #p <.05 in Scramble TGFb v.s. Scramble Veh (n = 6). (F) Cell migration assay results of scramble and INSRe5‐8 KO HSCs treated with TGF beta 5 ng/mL for 24 h. (G) Immunostaining with alpha ‐smooth muscle actin ( alpha SMA) antibody and DAPI for nucleus visualization in scramble and INSRe5‐8 KO HSCs treated with TGF beta 5 ng/mL for 24 h (*p <.05; **p <.01; ***p <.001, n = 3 each; two‐way ANOVA; ± SEM). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/40022609), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for HO-1/HMOX1/HSP32 Antibody

Application
Recommended Usage

Knockout Validated

HO‑1/HMOX1/HSP32 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in HO‑1/HMOX1/HSP32 knockout HeLa cell line.

Simple Western

25 µg/mL
Sample: A20 mouse B cell lymphoma cell line and A549 human lung carcinoma cell line

Western Blot

0.5 µg/mL
Sample: A549 human lung carcinoma cell line, DU145 human prostate carcinoma cell line, and A20 mouse B cell lymphoma cell line

Reviewed Applications

Read 1 review rated 5 using AF3776 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HO-1/HMOX1/HSP32

Heme Oxygenase 1 (HO-1), also known as HMOX1 and Heat Shock Protein 32 (HSP32), is a 32 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 (HO-1/HMOX1) and a constitutive heme oxygenase-2 (HO-2/HMOX2). HO-1 expression is induced by heme and other non-heme compounds. Human HO-1 shares 82% amino acid sequence identity with mouse HO-1.

Long Name

Heme Oxygenase 1

Alternate Names

HMOX1, HO1, HSP32

Entrez Gene IDs

3162 (Human); 24451 (Mouse); 15368 (Rat)

Gene Symbol

HMOX1

UniProt

P09601

Additional HO-1/HMOX1/HSP32 Products

Product Documents for HO-1/HMOX1/HSP32 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for HO-1/HMOX1/HSP32 Antibody

For research use only

Citations for HO-1/HMOX1/HSP32 Antibody

Customer Reviews for HO-1/HMOX1/HSP32 Antibody (1)

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  • HO-1 in A549
    Name: Anonymous
    Application: Simple Western
    Sample Tested: A549 human lung carcinoma cell line
    Species: Human
    Verified Customer | Posted 08/07/2024
    HO-1 in A549 after cigarette smoke treatment
    Milk-free diluent, 1:40
    HO-1/HMOX1/HSP32 Antibody AF3776

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