Lumican Antibody (669401)

R&D Systems | Catalog # MAB28461

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 669401
View all Lumican Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Lumican
Gln19-Asn338
Accession # NP_002336

Specificity

Detects human and mouse Lumican in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Lumican Antibody (669401)

Detection of Human Lumican antibody by Western Blot.

Detection of Human Lumican by Western Blot.

Western blot shows lysates of human cartilage tissue. PVDF Membrane was probed with 2 µg/mL of Human Lumican Monoclonal Antibody (Catalog # MAB28461) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Differentially glycosylated Lumican protein species were detected between 65 and 85 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.

Applications for Lumican Antibody (669401)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human cartilage tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lumican

Lumican is a secreted matrix protein that belongs to the small leucine‑rich proteoglycan (SLRP) class II family. Mature Lumican has a negatively charged N‑terminal domain that contains tyrosine sulfates, followed by twelve leucine‑rich repeats, which are flanked by cysteine loops. Lumican is expressed widely in mammalian connective tissues, while corneal Lumican exists as a keratan sulfate proteoglycan in adult cartilage, Lumican exists primarily as a glycoprotein and lacks keratan sulfate. The amino acid sequence of human Lumican shares 88% amino acid sequence identity with that of mature mouse Lumican.

Alternate Names

LDC, LUM, SLRR2D

Entrez Gene IDs

4060 (Human); 17022 (Mouse)

Gene Symbol

LUM

UniProt

NP_002336

Additional Lumican Products

Product Documents for Lumican Antibody (669401)

Certificate of Analysis

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Product Specific Notices for Lumican Antibody (669401)

For research use only

Related Research Areas

Proteoglycans

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