Human/Mouse MBD4 Alexa Fluor® 350-conjugated Antibody

Catalog #: AF5935U Datasheet / COA / SDS

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AF5935U-100UG

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Human/Mouse MBD4 Alexa Fluor® 350-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse MBD4 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human MBD4
Met382-Ser580
Accession # O95243

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 350 (Excitation= 346 nm, Emission= 442 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: MBD4

MBD4 (Methyl CpG-binding domain protein 4; also MED1) is a 65-68 kDa member of the methyl CpG binding protein family. It is expressed in non-germline cells, and serves at least two functions: one, it interacts with FADD to up- or down-regulate apoptosis, and two, it acts as a DNA N-glycosylase that excises improper chromosomal thymines that are created through the spontaneous deamination of methylcytosine to thymine. Human MDB4 is 580 amino acids (aa) in length. It contains one DNA-binding MBD domain (aa 76-148) and one endonuclease domain (aa 455-580). There are three isoform variants. One shows a deletion of aa 395-400, another shows an AlaPro substitution for aa 539-580, and a third shows a Gln substitution for aa 83-401. Over aa 382-580, human MBD4 shares 90% aa identity with mouse MBD4.

Long Name

Methyl-CpG Binding Domain Protein 4

Entrez Gene IDs

8930 (Human); 17193 (Mouse); 680915 (Rat)

Alternate Names

EC 3.2.2.-; G/T mismatch glycosylase; G/U mismatch glycosylase; MBD4; MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics; methyl-CpG binding domain protein 4; methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase; Methyl-CpG-binding endonuclease 1; Methyl-CpG-binding protein MBD4; Mismatch-specific DNA N-glycosylase; putative methyl-CpG binding protein