Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all MBD4 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human MBD4
Met382-Ser580
Accession # O95243

Specificity

Detects human and mouse MBD4 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse MBD4 Antibody

Detection of Human and Mouse MBD4 antibody by Western Blot.

Detection of Human and Mouse MBD4 by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse MBD4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5935) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for MBD4 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse MBD4 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Raji human Burkitt's lymphoma cell line, and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MBD4

MBD4 (Methyl CpG-binding domain protein 4; also MED1) is a 65-68 kDa member of the methyl CpG binding protein family. It is expressed in non-germline cells, and serves at least two functions: one, it interacts with FADD to up- or down-regulate apoptosis, and two, it acts as a DNA N-glycosylase that excises improper chromosomal thymines that are created through the spontaneous deamination of methylcytosine to thymine. Human MDB4 is 580 amino acids (aa) in length. It contains one DNA-binding MBD domain (aa 76-148) and one endonuclease domain (aa 455-580). There are three isoform variants. One shows a deletion of aa 395-400, another shows an AlaPro substitution for aa 539-580, and a third shows a Gln substitution for aa 83-401. Over aa 382-580, human MBD4 shares 90% aa identity with mouse MBD4.

Long Name

Methyl-CpG Binding Domain Protein 4

Alternate Names

EC 3.2.2.-, G/T mismatch glycosylase, G/U mismatch glycosylase, MED1G/5-fluorouracil mismatch glycosylase with biphasic kinetics, methyl-CpG binding domain protein 4, methyl-CpG-binding domain protein 43,N(4)-ethenocytosine glycosylase, Methyl-CpG-binding endonuclease 1, Methyl-CpG-binding protein MBD4, Mismatch-specific DNA N-glycosylase, putative methyl-CpG binding protein

Entrez Gene IDs

8930 (Human); 17193 (Mouse); 680915 (Rat)

Gene Symbol

MBD4

UniProt

O95243 (Human)

Additional MBD4 Products

Product Documents for Human/Mouse MBD4 Antibody

Certificate of Analysis

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Product Specific Notices for Human/Mouse MBD4 Antibody

For research use only

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