Human/Mouse Nrf2 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF3925T Datasheet / COA / SDS

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AF3925T-100UG

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Human/Mouse Nrf2 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human Nrf2 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse Nrf2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human Nrf2
Met17-Asn605
Accession # Q16236

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Nrf2

NF-E2 related factor 2 (Nrf2) is a member of the cap ‘n’ collar family of basic leucine zipper transcription factors. By binding antioxidant response elements (AREs), Nrf2 activates the transcription of genes encoding antioxidant and phase II drug-metabolizing enzymes needed to protect cells against oxidative damage. Transcriptional targets regulated by Nrf2 include genes encoding glutathione S-transferases, superoxide dismutase-1, and heme oxygenase-1.

Long Name

NF-E2-related Factor 2

Entrez Gene IDs

4780 (Human); 18024 (Mouse); 83619 (Rat)

Alternate Names

HEBP1; NFE2L2; NF-E2-related factor 2; NFE2-related factor 2; NRF2 erythroid derived 2, like 2; Nrf2; nuclear factor (erythroid-derived 2)-like 2; nuclear factor erythroid 2-related factor 2; nuclear factor erythroid-derived 2-like 2