Human/Mouse/Rat APBA2 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF6327R Datasheet / COA / SDS

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AF6327R-100UG

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Human/Mouse/Rat APBA2 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human APBA2 in direct ELISAs and human, mouse and rat in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) APBA1 and rhAPBA3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human APBA2
Ala2-Leu165
Accession # Q99767

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: APBA2

APBA2 (Adaptor protein X11 beta, also Mint2 and X11L) is a cytosolic member of the X11 family of adaptor proteins. Although its predicted MW is 83 kDa, it runs anomalously at 130-135 kDa in SDS-PAGE. APBA2 is expressed in neurons, and appears to play a role in A beta formation from APP. Normally, APBA2 associates with the cytoplasmic domain of transmembrane APP. This precludes its entry into lipid rafts, where it begins formation of A beta following cleaved by BACE. Human APBA2 is 749 amino acids (aa ) in length. It contains one phosphotyrosine binding (PTB) domain (aa 366-533) plus two PDZ domains (aa 568-734). Over aa 221-250, there is an APP interaction site that contains a key phosphorylation site at Ser238. There are two potential splice forms, one that shows a deletion of aa 406-417, and a second that contains a 44 aa substitution for aa 170-214. Over aa 1-165, human APBA2 shares 85% aa identity with mouse APBA2.

Long Name

Amyloid beta (A4) Precursor Protein-binding, Family A, Member 2

Entrez Gene IDs

321 (Human); 11784 (Mouse); 83610 (Rat)

Alternate Names

Amyloid-beta A4 precursor protein-binding family A member 2;D15S1518E;HsT16821;LIN-10;MGC:14091;MINT2;X11-BETA;X11L; APBA2; LIN-10; MINT2; X11L