Human/Mouse/Rat APBA2 Alexa Fluor® 700-conjugated Antibody

Catalog #: AF6327N Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF6327N-100UG

Save 15% on Select RUO Reagents. See Details.

All APBA2 Antibodies

Product Details

Supplemental Products

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat APBA2 Alexa Fluor® 700-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human APBA2 in direct ELISAs and human, mouse and rat in Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) APBA1 and rhAPBA3 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human APBA2
Ala2-Leu165
Accession # Q99767

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 700 (Excitation= 675-700 nm, Emission= 723 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: APBA2

APBA2 (Adaptor protein X11 beta, also Mint2 and X11L) is a cytosolic member of the X11 family of adaptor proteins. Although its predicted MW is 83 kDa, it runs anomalously at 130-135 kDa in SDS-PAGE. APBA2 is expressed in neurons, and appears to play a role in A beta formation from APP. Normally, APBA2 associates with the cytoplasmic domain of transmembrane APP. This precludes its entry into lipid rafts, where it begins formation of A beta following cleaved by BACE. Human APBA2 is 749 amino acids (aa ) in length. It contains one phosphotyrosine binding (PTB) domain (aa 366-533) plus two PDZ domains (aa 568-734). Over aa 221-250, there is an APP interaction site that contains a key phosphorylation site at Ser238. There are two potential splice forms, one that shows a deletion of aa 406-417, and a second that contains a 44 aa substitution for aa 170-214. Over aa 1-165, human APBA2 shares 85% aa identity with mouse APBA2.

Long Name

Amyloid beta (A4) Precursor Protein-binding, Family A, Member 2

Entrez Gene IDs

321 (Human); 11784 (Mouse); 83610 (Rat)

Alternate Names

Amyloid-beta A4 precursor protein-binding family A member 2;D15S1518E;HsT16821;LIN-10;MGC:14091;MINT2;X11-BETA;X11L; APBA2; LIN-10; MINT2; X11L