Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 461908
View all CDC25C Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CDC25C
Ser2-Pro473
Accession # P30307

Specificity

Detects human, mouse and rat CDC25C in Western blots. In Western blots, no cross-reactivity with recombinant human (rh) CDC25A or rhCDC25B is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat CDC25C Antibody

Detection of Human and Mouse CDC25C antibody by Western Blot.

Detection of Human and Mouse CDC25C by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Rat Anti-Human/Mouse/Rat CDC25C Monoclonal Antibody (Catalog # MAB4459) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for CDC25C at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of CDC25C by Western Blot

Detection of CDC25C by Western Blot

Knockdown ANXA1 Induces G2/M Cell Cycle Arrest in Glioma Cells via the PI3K/Akt Signaling Pathway. A: Inhibition of ANXA1 decreases the expression of cdc25C, cyclin B1 and cdc2, whereas cyclin D1 was not altered evidently. Western blot assay analysis was performed using anti-cdc25C, anti-cyclin B1, anti-cyclin D1, anti-beta -actin and anti-cdc2 antibodies. B: The relative amounts of cdc-25C, cyclin B1, cdc2 and Cyclin D1 were quantified by a densitometric analysis (ImageJ). C: Western blot analysis of PI3K subunit p110 alpha and p85, phosphorylation and total protein levels of Akt, GSK3 beta and beta -catenin after si-ANXA1 or si-NC transfection for 48 h. D: Quantitative charts of phosphorylation and the total level of Akt, PI3K subunit p110 alpha and p85, GSK3 beta and beta -catenin. E: Inhibition of ANXA1 has no effect on the expression of p-p65NF-kappa B and up-regulates the expression of cPLA2. Western blot assay analysis was performed using anti-p-p65NF-kappa B, anti-p65NF-kappa B, anti-cPLA2 and anti-beta -actin antibodies. F: The relative amounts of p-p65NF-kappa B and cPLA2 were quantified by a densitometric analysis (ImageJ). G: ELISA analysis of nuclear NF-kappa B activity. N = 9 per group. H: cPLA2 activity in control, si-NC and si-ANXA1 U87 cells. cPLA2 activity was determined as described in Methods. These results were expressed as the mean ± SD from three independent experiments, *p < 0.05, **p < 0.01 compared with si-NC group. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33706561), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human/Mouse/Rat CDC25C Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, CHP-100 human neuroblastoma cell line, and C2C12 mouse myoblast cell line

Reviewed Applications

Read 1 review rated 4 using MAB4459 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CDC25C

Cell Division Cycle 25C (CDC25C) is a dual serine/threonine and tyrosine phosphatase. There are four splice variants with molecular weights of 46, 47, 49, and 53 kDa. The smaller variants lack key regulatory sites and their upregulation has been observed in prostate cancer. CDC25C regulates the G2/M cell cycle transition by dephosphorylating proteins such as Chk1. Its cellular distribution is controlled by phosphorylations that enhance 14-3-3 binding, block nuclear localization, and inhibit enzymatic activity.

Long Name

Cell Division Cycle 25C

Alternate Names

CDC 25, CDC25, cell division cycle 25 homolog C (S. cerevisiae), cell division cycle 25 homolog C (S. pombe), cell division cycle 25C, Dual specificity phosphatase Cdc25C, EC 3.1.3.48, mitosis inducer CDC25, M-phase inducer phosphatase 3, phosphotyrosine phosphatase

Entrez Gene IDs

995 (Human); 12532 (Mouse); 307511 (Rat)

Gene Symbol

CDC25C

UniProt

P30307

Additional CDC25C Products

Product Documents for Human/Mouse/Rat CDC25C Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat CDC25C Antibody

For research use only

Related Research Areas

Cell Cycle
Phosphatases and Regulators

Citations for Human/Mouse/Rat CDC25C Antibody

Customer Reviews for Human/Mouse/Rat CDC25C Antibody (1)

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  • Human/Mouse/Rat CDC25C Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Human
    Verified Customer | Posted 01/25/2018
    Human/Mouse/Rat CDC25C Antibody MAB4459

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