DRAK2 Antibody (685228)

R&D Systems | Catalog # MAB6640

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 685228
View all DRAK2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human DRAK2
Ala87-Glu285 (predicted)
Accession # O94768

Specificity

Detects human, mouse, and rat DRAK2 in Western blots. In Western blots, less than 10% cross-reactivity with recombinant human (rh) DRAK1, rhDAPK1, rhDAPK2, or rhDAPK3 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for DRAK2 Antibody (685228)

Detection of Human, Mouse, and Rat DRAK2 antibody by Western Blot.

Detection of Human, Mouse, and Rat DRAK2 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF Membrane was probed with 1 µg/mL of Human DRAK2 Monoclonal Antibody (Catalog # MAB6640) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DRAK2 at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Applications for DRAK2 Antibody (685228)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DRAK2

DRAK2 (death-associated protein related kinase-2), also called STK17B (Ser/Thr kinase 17B) is a 45 kDa member of the DAPK (death-associated protein kinase) family. It is expressed mainly in lymphoid organs by mature T cells and B cells. It is autophosphorylated on Ser12 after TCR stimulation and negatively regulates TCR signaling. Ultraviolet light causes phosphorylation on Ser350 and translocation to the nucleus to stimulate apoptosis. Within the region used as an immunogen, human DRAK2 shares 95% amino acid (aa) identity with mouse and rat DRAK2 while mouse and rat DRAK2 share 99.5% aa identity.

Long Name

DAP Kinase-related Apoptosis-inducing Protein Kinase 2

Alternate Names

STK17B

Entrez Gene IDs

9262 (Human)

Gene Symbol

STK17B

UniProt

O94768

Additional DRAK2 Products

Product Documents for DRAK2 Antibody (685228)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for DRAK2 Antibody (685228)

For research use only

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