FK506 binding protein, 13 kDa molecular weight (FKBP13), also called FKBP2, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressant FK506 is a potent FKBP13 inhibitor. FKBP13 is strongly induced by calcium ionophores and agents that cause endoplasmic reticulum stress, such as tunicamycin. FKBP13 is membrane-associated and tightly bound to the cytoskeletal protein Band 4.1 in erythrocytes, suggesting a role in maintaining structural proteins.
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # 422617
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Product Specifications
Immunogen
E. coli-derived recombinant human FKBP13
Ala22-Leu142
Accession # P26885
Ala22-Leu142
Accession # P26885
Specificity
Detects endogenous human, mouse, and rat FKBP13 at 13 kDa in Western blots. In Western blots, this antibody does not cross-react with recombinant human FKBP12, FKBP12.6, FKBP25, FKBP38, FKBP51, or FKBP52.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for FKBP13 Antibody (422617)
Detection of Human and Rat FKBP13 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP13 Monoclonal Antibody (Catalog # MAB4356) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FKBP13 at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for FKBP13 Antibody (422617)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: MCF-7 human breast cancer cell line, PC-12 rat adrenal pheochromocytoma cell line, and Rat-2 rat embryonic fibroblast cell line
Reviewed Applications
Read 2 reviews rated 3.5 using MAB4356 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: FKBP13
Long Name
13 kDa FK506 Binding Protein
Alternate Names
FKBP2
Gene Symbol
FKBP2
UniProt
Additional FKBP13 Products
Product Documents for FKBP13 Antibody (422617)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for FKBP13 Antibody (422617)
For research use only
Related Research Areas
Citations for FKBP13 Antibody (422617)
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Customer Reviews for FKBP13 Antibody (422617) (2)
3.5 out of 5
2 Customer Ratings
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
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- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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