GRK2 Antibody (2089B)

Detection of GRK2 by Western Blot

Validation of CRISPR/Cas9 knock-out cell lines. (a) Western blot analysis of full-length GRK2 or GRK3 expression in parental or genome-edited cell lines with deletion of GRK2, GRK3 or GRK2/3. The anti-GRK2 and anti-GRK3 antibodies target a site C-terminal to the site that is genetically modified. GAPDH expression is detected to ensure equal loading. Full-length blots are presented in Supplementary Fig. 1. (b) Quantification of anti-GRK2 and anti-GRK3 western blots after normalization to the GAPDH signal. Mean ± SEM of 4–8 independent experiments. Expression in each of the KO cell lines was compared to the parental cells by one sample t-test using a reference value of 100 and the cutoff for what was considered a significant difference was adjusted to P < 0.05/n (n corresponds to the number of observations) to correct for multiple comparisons. ***P < 0.001/n. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33060647), licensed under a CC-BY license. Not internally tested by R&D Systems.