HSP90 Antibody (341320)

R&D Systems | Catalog # MAB3286

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Chicken, Fish - Danio rerio (Zebrafish)

Applications

Validated:

Western Blot, Simple Western

Cited:

Western Blot, Simple Western, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 341320
View all HSP90 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human HSP90 beta
Met1-Asp724
Accession # P08238

Specificity

Detects human, mouse and rat HSP90 in direct ELISAs and Western blots. Reacts with both recombinant human HSP90 alpha and HSP90 beta isoforms in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for HSP90 Antibody (341320)

Detection of Human/Mouse/Rat HSP90 antibody by Western Blot.

Detection of Human/Mouse/Rat HSP90 by Western Blot.

Western blot shows lysates of Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.25 µg/mL Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human HSP90a and HSP90 beta (5 ng/lane) were included. A specific band for HSP90 was detected at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.
Detection of Human and Rat HSP90 antibody by Simple WesternTM.

Detection of Human and Rat HSP90 by Simple WesternTM.

Simple Western lane view shows lysates of Raji human Burkitt's lymphoma cell line and L6 rat myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HSP90 at approximately 95 kDa (as indicated) using 2.5 µg/mL of Mouse Anti-Human/Mouse/Rat HSP90 Monoclonal Antibody (Catalog # MAB3286). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of HSP90 by Western Blot

Detection of HSP90 by Western Blot

Nup358 is required for HDAC3 function and nuclear translocation.(A) Upstream transcriptional regulators (P < 0.05) predicted to be altered in Nup358 knockout LSK cells belonging to cluster 4 and cluster 9 ranked by P value. (B) The intracellular localization of HDAC3, E2F1, RB1, p53, RBL2, DMTF1, and GTF2I was analyzed by immunofluorescence in 293T cells transfected with control or Nup358-specific siRNAs. Scale bars, 25 μm. (C) HDAC3 intracellular localization was analyzed by immunofluorescence in 293T cells transfected with control or Nup358-specific siRNAs. Cells were costained with antibodies against nucleoporins Nup358 and Nup98. Scale bars, 25 μm. (D) Quantification of HDAC3 nuclear/cytoplasmic signal ratio in control or Nup358 knockdown cells. Data are expressed as mean ± SD. ****P ≤ 0.0001. (E) Protein levels of HDAC3 and Nup358 in cells transfected with scramble control or Nup358-specific siRNAs were analyzed by Western blot. HSP90 was used as loading control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38838144), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of HSP90 by Western Blot

Detection of HSP90 by Western Blot

Modulatory effects of SGME on the Nrf2/HO-1 signaling pathway. (A–D) Raw 264.7 macrophages were pretreated with SGME for 3 h and then stimulated with LPS for 1 h. (A and B) Protein levels of Nrf2 in nuclear and cytoplasmic lysates were examined by western blot. (C and D) Nrf2 was labeled by immunostaining. Representative images showed the distribution of Nrf2 (green) in relation to nuclei (red); the bar graph depicted relative nuclear fluorescence intensity of Nrf2 calculated by ImageJ; scale bar: 20 μm. (E–G) After treatment with SGME for 3 h, Raw 264.7 macrophages were treated with LPS for 3 h. Protein and mRNA levels of HO-1 were measured by western blot and RT-qPCR. * denotes p < 0.05; n = 3.– Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/42031984), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of HSP90 by Western Blot

Detection of HSP90 by Western Blot

Roles of Nrf2/HO-1 in modulation of inflammatory responses by SGME. (A) Raw 264.7 cells were sequentially treated with ML385 (10 µM) or SnPP (10 µM) (pharmacological inhibitors of Nrf2 and HO-1, respectively) for 1 h, SGME (30 µg/mL) for 3 h, and LPS (100 ng/mL) for 24 h. Nitrite/nitrate levels were measured in culture medium. (B–H) Cells were transfected with an siRNA targeting Nrf2 (20 nM) or a scramble siRNA for 24 h. Then, cells were treated with SGME (30 µg/mL) for 3 h, followed by LPS (100 ng/mL) for 24 h (B), 6 h (C–F), or 1 h (G–H). (B) NO production was determined by measuring nitrite/nitrate release into culture medium. (C–F) The mRNA levels of genes encoding iNOS (C), IL-6 (D), COX-2 (E), and TNF-alpha (F) were measured using RT-qPCR. (G and H) The expression of p65 NF-kappa B in cytoplasmic and nuclear fractions was examined by western blot. (I and J) BMDMs were treated with ML385 (10 µM) or SnPP (10 µM) for 1 h and then with SGME (30 µg/mL) for an additional 3 h. BMDMs were next incubated with LPS/IFN-gamma (100/10 ng/mL) for 24 h to induce M1 polarization. The expression of CD86 (I) and CD80 (J) on the cell surface was analyzed using flow cytometry. * denotes p < 0.05; ns: statistically non-significant; n = 3. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/42031984), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for HSP90 Antibody (341320)

Application
Recommended Usage

Simple Western

2.5 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and L6 rat myoblast cell line

Western Blot

0.25 µg/mL
Sample: Raji human Burkitt's lymphoma cell line and HeLa human cervical epithelial carcinoma cell line, CH-1 mouse B cell lymphoma cell line, A20 mouse B cell lymphoma cell line, and L6 rat myoblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HSP90

HSP90 is a composite name for a large group of Heat Shock Proteins whose molecular weights average 90 kDa. HSPs functions primarily as a molecular chaperone, facilitating the folding of other cellular proteins, preventing protein aggregation, or targeting improperly folded proteins to specific degradative pathways. HSP90 is a central component in a number of basic cellular processes including hormone signaling and cell cycle control. HSP90 is ubiquitously expressed, highly conserved and accounts for 1‑2% of the total cellular protein. Recently introduced, standardized nomenclature has divided the 17 identified HSP90 genes into three related and one unrelated classes, HSP90AA, HSP90AB, HSP90BB, and TRAP, respectively. Eukaryotic cells have two principal isoforms of HSP90. The antibody described here reacts with the 724 amino acid constitutively expressed HSP90AB1-isoform 1 (also known as HSP90 beta, HSP90B, HSPCB, HSPC2, and HSP89 beta ) and also with the 737 amino acid inducible form, HSP90AA1 (also known as HSP90 alpha, HSP90A, HSPC1, HSPCA, HSP89 alpha and LAP2). HSP90AB1-1 and HSP90AA share 90% identity.

Long Name

Heat Shock Protein 90

Alternate Names

FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38

UniProt

P08238

Additional HSP90 Products

Product Documents for HSP90 Antibody (341320)

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Product Specific Notices for HSP90 Antibody (341320)

For research use only

Citations for HSP90 Antibody (341320)

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Associated Pathways

Apoptosis Signaling Pathway
Apoptosis Signaling Pathway Thumbnail