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Human/Mouse/Rat Lipin 2 Antibody

Catalog #: AF3756
1 Citation Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF3756
AF3756-SP

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Citations (1)
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Human/Mouse/Rat Lipin 2 Antibody Summary

Species Reactivity
Human, Mouse, Rat
Specificity
Detects Lipin 2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) Lipin 1 and rhLipin 3 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Lipin 2
Ser439-Lys563
Accession # Q92539
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human Lipin 2

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipin 2

Human Lipin 2 is a 100 kDa (896 aa) nuclear protein that belongs to the large, evolutionarily conserved Lipin family. All Lipin family members have a conserved N‑lipin domain at the N‑termini, a nuclear localization signal, and a conserved C-lipin domain near the C-termini. The functions of the N- and C-lipin domain are not known. Lipin 2 is widely expressed. Mutations in human Lipin 2 have been associated with autosomal dominant high grade myopia and Majeed syndrome. Over the region used as immunogen, human and mouse Lipin 2 are 100% identical. Lipin 2 also shares approximately 48% - 50% amino acid sequence identity with Lipin 1 and Lipin 3.

Long Name
Phosphatidate Phosphatase LPIN2
Entrez Gene IDs
9663 (Human); 64898 (Mouse); 316737 (Rat)
Alternate Names
EC 3.1.3.4; KIAA0249lipin-2; Lipin 2; Lipin-2; LPIN2; phosphatidate phosphatase LPIN2

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Citation for Human/Mouse/Rat Lipin 2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Dietary cholesterol reduces plasma triacylglycerol in apolipoprotein e-null mice: suppression of lipin-1 and -2 in the glycerol-3-phosphate pathway.
    Authors: Obama T, Nagaoka S, Akagi K, Kato R, Horiuchi N, Horai Y, Aiuchi T, Arata S, Yamaguchi T, Watanabe M, Itabe H
    PLoS ONE, 2011-08-09;6(8):e22917.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Tissue Homogenates
    Applications: Western Blot

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