Human/Mouse/Rat Neurexophilin-3/NXPH-3 Antibody

Catalog #: AF5098 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5098
AF5098-SP

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Detection of Human Neurexophilin‑3/NXPH‑3 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat Neurexophilin-3/NXPH-3 Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, and rat Neurexophilin-3/NXPH-3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human Neurexophilin-1/NXPH1 and recombinant rat Neurexophilin-1/NXPH1 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neurexophilin-3/NXPH-3
Gln23-Gly252
Accession # AAI66603

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Neurexophilin‑3/NXPH‑3 by Western Blot. Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Neurexophilin-3/NXPH-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5098) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Neurexophilin-3/NXPH-3 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurexophilin-3

Neurexophilin-3 (NXPH-3) is a neuropeptide-like secreted glycoprotein in the neurexophilin family (1, 2). The 252 amino acid (aa) NXPH-3 precursor contains a 22 aa signal peptide, plus a 230 aa proprecursor that is likely cleaved at a basic motif, producing a 76 aa propeptide and a 154 aa mature protein (1). Mature rat NXPH-3 shares 99%, 95% and 92% aa identity with mouse, human and bovine NXPH-3, respectively. It contains motifs that are conserved among all neurexophilins, including three potential N-glycosylation sites in the N-terminal portion and six cysteines in the C-terminal portion (1). NXPH-3 is expressed selectively in subplate-derived neurons in the cortex, granule cells in the vestibulocerebellum, and Cajal-Retzius cells during development (3). NXPH-1 is the neurexophilin most similar to NXPH-3, sharing 69% aa identity within the mature region. Expression of NXPH-1 and NXPH-3 does not appear to overlap, with NXPH-1 expression occurring mainly by cells that resemble inhibitory interneurons (2-4). Both are tightly bound extracellular ligands of alpha -neurexins, synaptic transmembrane molecules that are essential for calcium-triggered neurotransmitter release (1, 5, 6). Genetic deletion of NXPH-1 and/or NXPH-3 produces no anatomical effect, although mice lacking NXPH-3 show specific defects in motor coordination (3, 4). Of the other known neurexophilins, NXPH-2 is not expressed in rodents, and NXPH-4 does not bind alpha -neurexins (1, 5).

References