Human/Mouse/Rat Nrf1 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB5306S Datasheet / COA / SDS

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FAB5306S-100UG

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Human/Mouse/Rat Nrf1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects endogenous human, mouse and rat Nrf1 at 85 kDa in Western blots.

Source

Monoclonal Mouse IgG2a Clone # 492413

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Nrf1
Met1-Lys742
Accession # AAH10623

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Nrf1

NF-E2-related factor 1 (Nrf1; also transcription factor 11 and LCR-F1) is a ubiquitously expressed transcription factor and member of the bZIP family and the CNC subfamily. Human Nrf1 is 772 amino acids (aa) in length. Like other members of the bZIP family, Nrf1 contains heptad repeats of leucine and hydrophobic residues within a putative amphipathic helical domain of 40 aa and is preceded by a 30 aa domain rich in arginine and lysine residues. A splicing variant produces two isoforms for Nrf1. Isoform 2 has a 30 aa deletion corresponding to aa 242‑271 in isoform 1. Human Nrf1 is 96% and 93% identical to bovine and mouse Nrf1, respectively.

Long Name

NF-E2-related Factor 1

Entrez Gene IDs

4779 (Human); 18023 (Mouse); 360610 (Rat)

Alternate Names

Alpha palindromic-binding protein; ALPHA-PAL; EWG; HBZ17; LCR-F1; NFE2L1; Nrf1; NRF-1; nuclear respiratory factor 1; TCF11