Human/Mouse/Rat PCK1 Alexa Fluor® 594-conjugated Antibody

Catalog #: AF7316T Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF7316T-100UG

Save 15% on Select RUO Reagents. See Details.

All PCK1 Antibodies

Product Details

Reviews

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Mouse/Rat PCK1 Alexa Fluor® 594-conjugated Antibody Summary

Species Reactivity

Human, Mouse, Rat

Specificity

Detects human, mouse, rat PCK1 in Western blots, and detects recombinant human PCK1 in direct ELISAs. In direct ELISAs, less than 3% cross-reactivity with recombinant mouse PCK1 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PCK1
Met1-Ile88
Accession # P35558

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 594 (Excitation= 590 nm, Emission= 617 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: PCK1

PCK1 (Phosphoenolpyruvate carboxykinase, cytosolic; also PEPCK-C) is a 67-69 kDa cytosolic member of the phosphoenolpyruvate kinase (GTP) family of enzymes. It is found in hepatocytes, adipocytes, intestinal and renal proximal tubule epithelial cells. PCK1 is one of two enzymes that drive the conversion of oxaloacetate plus GTP into PEP, GDP and CO₂. The PEP generated in this reaction forms glucose via fructose phosphate intermediates. The other enzyme that has this activity is termed PEPCK-M and found in mitochondria. Human PCK1 is 622 amino acids (aa) in length. It contains a large catalytic region (aa 27-615) and three utilized acetylation sites. There is one potential alternative start site at Met315. Over aa 1-88, human PCK1 shares 80% aa sequence identity with mouse PCK1.

Long Name

Phosphoenolpyruvate Carboxykinase 1

Entrez Gene IDs

5105 (Human); 18534 (Mouse); 362282 (Rat)

Alternate Names

EC 4.1.1.32; MGC22652; PCK1; PEPCK1; PEPCKC; PEPCK-C; PEPCK-CPEP carboxykinase; phosphoenolpyruvate carboxykinase 1 (soluble); phosphoenolpyruvate carboxykinase, cytosolic [GTP]; phosphoenolpyruvate carboxykinase, cytosolic; Phosphoenolpyruvate carboxylase; phosphopyruvate carboxylase