Human/Mouse/Rat PKLR Antibody

R&D Systems | Catalog # AF8519

R&D Systems
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Simple Western

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all PKLR Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKLR
Leu47-Ser574
Accession # P30613

Specificity

Detects human, mouse, and rat PKLR in direct ELISAs and Western blots.  In direct ELISAs, less than 1% cross-reactivity with recombinant human PKM-2 is observed.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat PKLR Antibody

Detection of Human, Mouse, and Rat PKLR antibody by Western Blot.

Detection of Human, Mouse, and Rat PKLR by Western Blot.

Western blot shows lysates of human liver tissue, mouse liver tissue, and rat liver tissue. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat PKLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8519) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PKLR at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human and Mouse PKLR by Simple WesternTM.

Simple Western lane view shows lysates of human liver and mouse liver, loaded at 0.2 mg/mL. A specific band was detected for PKLR at approximately 57 kDa (as indicated) using 0.5 µg/mL of Rabbit Anti-Human/Mouse/Rat PKLR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8519). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human/Mouse/Rat PKLR Antibody

Application
Recommended Usage

Simple Western

0.5 µg/mL
Sample: Human Liver and Mouse Liver

Western Blot

1:1000 dilution
Sample: Human liver tissue, mouse liver tissue, and rat liver tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

For liquid material, refer to CoA for concentration.

Formulation

Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: PKLR

Human PKLR (Pyruvate kinase PKLR) is a 60 kDa, cytosolic red cell/liver pyruvate kinase that belongs to the pyruvate kinase family of proteins.  It is 574 amino acids (aa) in length, and contains two Allosteric activator binding regions. PKLR is allosterically activated by fructose 1,6-bisphosphate. Pyruvate kinase-deficient patients suffer from a highly variable degree of chronic hemolysis, ranging from severe neonatal jaundice and fatal anemia at birth, severe transfusion-dependent chronic hemolysis, moderate hemolysis with exacerbation during infection, to a fully compensated hemolysis without apparent anemia. Additional shorter isoform apparently exists. It is 543 aa in length and shows a two aa substitution for aa's 1-33. Human PKLR is 93% aa identical to mouse and rat PKLR.

Long Name

Pyruvate Kinase, Liver And RBC

Alternate Names

PK1, PKL, Pyruvate Kinase 1, RPK

Entrez Gene IDs

5313 (Human); 18770 (Mouse)

Gene Symbol

PKLR

UniProt

P30613

Additional PKLR Products

Product Documents for Human/Mouse/Rat PKLR Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human/Mouse/Rat PKLR Antibody

* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

For research use only

Customer Reviews for Human/Mouse/Rat PKLR Antibody

There are currently no reviews for this product. Be the first to review Human/Mouse/Rat PKLR Antibody and earn rewards!

Have you used Human/Mouse/Rat PKLR Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies