PKN2 Antibody (509105)

R&D Systems | Catalog # MAB5686

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 509105
View all PKN2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PKN2
Asp18-Asn207
Accession # Q16513

Specificity

Detects endogenous human, mouse and rat PKN2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for PKN2 Antibody (509105)

Detection of Human/Mouse/Rat PKN2 antibody by Western Blot.

Detection of Human/Mouse/Rat PKN2 by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, TF-1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKN2 Monoclonal Antibody (Catalog # MAB5686) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PKN2 at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for PKN2 Antibody (509105)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: A431 human epithelial carcinoma cell line, TF-1 human erythroleukemic cell line, C2C12 mouse myoblast cell line, NIH-3T3 mouse embryonic fibroblast cell line, L6 rat myoblast cell line, and Rat-2 rat embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PKN2

Serine/Threonine Protein Kinase N2 (PKN2), also known as PRK2, is a cytoplasmic kinase that contains three HR1 repeats (aa 44‑295), one C2 domain (aa 330‑463), and one protein kinase domain (aa 657‑916). PKN2 is a downstream effector of RhoA and promotes the activation of several kinases involved in regulation of the actin cytoskeleton, cell cycle, and apoptosis. PKN2 cleavage during apoptosis generates a C‑terminal fragment that inhibits PDK1 and Akt. PKN2 is also important in the intracellular replication of hepatitis C virus and Yersinia pestis.

Long Name

Protein Kinase N2

Alternate Names

PAK2, PRK2, PRKCL2, PRO2042

Entrez Gene IDs

5586 (Human); 109333 (Mouse); 207122 (Rat)

Gene Symbol

PKN2

UniProt

Q16513

Additional PKN2 Products

Product Documents for PKN2 Antibody (509105)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for PKN2 Antibody (509105)

For research use only

Related Research Areas

Diabetes
Intracellular Kinases

Citations for PKN2 Antibody (509105)

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