Human/Mouse/Rat SHP-2 Alexa Fluor® 532-conjugated Antibody

Applications

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Preparation and Storage

Background: SHP-2

Src-Homology domain-2 containing protein tyrosine Phosphatase 2 (SHP-2), also called protein tyrosine phosphatase, non-receptor type 11 (PTPN11), PTP1D, PTP2C, and SYP, is an enzyme that dephosphorylates tyrosine residues in proteins. The protein contains two Src homology 2 (SH2) domains, which both regulate the activity of the enzyme (1) and allow it to selectively bind to SH2 sites on proteins such as Dok1, IRS1, and the insulin receptor (2). SHP-2 plays a unique stimulatory role in cell signaling. Cells lacking SHP-2 have poor mobility because the hyper-phosphorylation of FAK and other proteins in the focal adhesion complex (3) prevents turnover of cellular attachment points. Without SHP-2, sustained ERK stimulation does not take place (4). The Y992 phosphorylation site of EGFR is a particularly good substrate for SHP-2 (5) and a phosphopeptide containing this sequence can be used to measure the activity of the enzyme (R&D Systems, Catalog # ES006) by detecting release of phosphate (R&D Systems, Catalog # DY996).

Product Datasheets

Related Research Areas

• Adhesion Molecules on Endothelial Cells
• Adhesion Molecules on VSMC
• C-type Lectin Receptors
• Epigenetics
• Focal Adhesions
• ITIM/ITAM Immunoreceptors and Related Molecules
• Mesenchymal to Epithelial Transition
• Negative Regulators of the Jak/STAT Pathway
• PDGF Family
• Phosphatases and Regulators
• Phosphatases in the Akt Pathway
• Platelet Signaling
• Repulsive Guidance Molecules and Receptors
• Search Kinases and Phosphatases
• VEGF Family