ROR gamma/RORC/NR1F3 Antibody (600214)

R&D Systems | Catalog # MAB6109

R&D Systems
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Western Blot

Cited:

Western Blot, Flow Cytometry, CyTof

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 600214
View all ROR gamma/RORC/NR1F3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ROR gamma /RORC/NR1F3
Met1-Gln100
Accession # P51449-2

Specificity

Detects human and mouse ROR gamma /RORC/NR1F3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for ROR gamma/RORC/NR1F3 Antibody (600214)

Detection of Human and Mouse ROR?/NR1F3 antibody by Western Blot.

Detection of Human and Mouse ROR gamma /NR1F3 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, EL-4 mouse lymphoblast cell line, and mouse thymus tissue. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse ROR gamma/NR1F3 Monoclonal Antibody (Catalog # MAB6109) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROR gamma/NR1F3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of ROR gamma/RORC/NR1F3 by Western Blot

Detection of ROR gamma/RORC/NR1F3 by Western Blot

Overexpression of EGR2 inhibits Th17 cell differentiation induced by the YAP/HIF-1 alpha /miR-182 signaling. a Th17 cell proportion after different treatments determined by flow cytometry; b the level of IL-17A in cell supernatant after different treatments measured by ELISA; c ROR gamma t mRNA expression in cells after different treatments determined by RT-qPCR; d Western blot analysis of ROR gamma t protein in cells after different treatments. Comparisons between multiple groups were conducted by one-way ANOVA with Tukey’s post hoc test; the experiments were repeated 3 times independently; * p < 0.05, compared with the cells treated with oe-NC; # p < 0.05, compared with the cells treated with oe-YAO or oe-HIF-1 alpha Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33980319), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of ROR gamma/RORC/NR1F3 by Western Blot

Detection of ROR gamma/RORC/NR1F3 by Western Blot

Increased YAP, HIF-1 alpha and miR-182 and decreased HDL-C level identified in asthma. a Th17 cell proportion in human PBMCs and mouse spleen cells detected by flow cytometry; b the serum level of IL-17A in asthma patients measured by ELISA; c ROR gamma t mRNA expression in human PBMCs determined by RT-qPCR; d Western blot analysis of ROR gamma t protein in human PBMCs; e mRNA expression of YAP and HIF-1 alpha in human PBMCs determined by RT-qPCR; f Western blot analysis of YAP and HIF-1 alpha proteins in human PBMCs; g miR-183/96/182 expression in human PBMCs determined by RT-qPCR. Comparisons between two groups were conducted by unpaired t test; * p < 0.05, compared with the normal individuals (normal); n = 22 in normal individuals; n = 29 in asthma patients. Each experiment was repeated 3 times independently Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33980319), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for ROR gamma/RORC/NR1F3 Antibody (600214)

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, EL‑4 mouse lymphoblast cell line, and mouse thymus tissue

Reviewed Applications

Read 2 reviews rated 5 using MAB6109 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ROR gamma/RORC/NR1F3

ROR gamma (Nuclear receptor ROR-gamma), also called RORC and NR1F3, is a member of the NR1 nuclear hormone receptor family. ROR gamma is a DNA binding transcription factor. ROR gamma is 518 amino acids (aa) in length. Deletion in mice implicates ROR gamma as being essential for lymphoid organogenesis and controlling apoptosis during thymopoiesis. Two splice forms differing in the first 24 aa have been found for this gene; isoform 2 deletes aa 1‑21 resulting in an alteration of aa 22‑24. Over aa 1‑100 human RORC shares 96% identity with mouse ROR gamma.

Long Name

Retinoic Acid-Related Orphan Receptor gamma

Alternate Names

NR1F3, RORC, RZRG

Entrez Gene IDs

6097 (Human)

Gene Symbol

RORC

UniProt

P51449-2

Additional ROR gamma/RORC/NR1F3 Products

Product Documents for ROR gamma/RORC/NR1F3 Antibody (600214)

Certificate of Analysis

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Product Specific Notices for ROR gamma/RORC/NR1F3 Antibody (600214)

For research use only

Citations for ROR gamma/RORC/NR1F3 Antibody (600214)

Customer Reviews for ROR gamma/RORC/NR1F3 Antibody (600214) (2)

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  • Human/Mouse ROR gamma /RORC/NR1F3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Thymus tissue
    Species: Mouse
    Verified Customer | Posted 12/20/2021
    ROR gamma/RORC/NR1F3 Antibody (600214) MAB6109
  • Human/Mouse ROR gamma /RORC/NR1F3 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: immune cells from lung
    Species: Mouse
    Verified Customer | Posted 12/05/2020
    ROR gamma/RORC/NR1F3 Antibody (600214) MAB6109

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FAQs for ROR gamma/RORC/NR1F3 Antibody (600214)

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  • Q: Does this ROR gamma/RORC/NR1F3 antibody come in lyophilized form?

    A: Yes, we do carry this RORC antibody in lyophilized format.

  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A sequence from Met1 - Gln100.

  • Q: Does this ROR gamma/RORC/NR1F3 antibody come in lyophilized form?

    A: Yes, we do carry this RORC antibody in lyophilized format.

  • Q: What is the immunogen sequence of this ROR gamma/RORC/NR1F3 antibody?

    A: A sequence from Met1 - Gln100.

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