Human/Mouse SAM68 Alexa Fluor® 488-conjugated Antibody

Catalog #: AF5509G Datasheet / COA / SDS

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AF5509G-100UG

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Human/Mouse SAM68 Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects human and mouse SAM68 in Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human and mouse SAM68
Met108-Pro280
Accession # Q07666

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: SAM68

SAM68 (Src associated in mitosis 68 kDa; also KH domain-containing, RNA-binding, signal transduction-associated protein 1) is a 62‑68 kDa member of the KHDRBS family of proteins. It is associated with multiple receptor systems (i.e.- TCR and leptin), as an adaptor protein by its SH2 and SH3 domains, and binds to UAAA motifs in mRNA. Human SAM68 is 443 amino acids (aa) in length. It contains one SGQ region (aa 96‑157) and one KH domain (aa 171‑197). Post‑translational modification of SAM68 alters its partnership with other molecules. For example, methylation on ArgGly motifs reduce RNA binding, acetylation on multiple Lys residues increases RNA binding, and phosphorylation on Tyr promotes SH3 domain interaction.

Long Name

Src-associated in Mitosis 68 kDa Protein

Entrez Gene IDs

10657 (Human); 20218 (Mouse); 117268 (Rat)

Alternate Names

FLJ34027; GAP-associated tyrosine phosphoprotein p62; KH domain containing, RNA binding, signal transduction associated 1; KH domain-containing, RNA-binding, signal transduction-associated protein 1; KHDRBS1; p21 Ras GTPase-activating protein-associated p62; p62; p68; SAM68; Sam68GAP-associated tyrosine phosphoprotein p62 (Sam68); Src-associated in mitosis 68 kDa protein