Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot, ELISA Capture (Matched Antibody Pair)

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 133931
View all sFRP-3 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human sFRP-3
Ala33-Asn325
Accession # AAB51298

Specificity

Detects human and mouse sFRP-3 in ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for sFRP-3 Antibody (133931)

Human sFRP-3 ELISA Standard Curve

Human sFRP-3 ELISA Standard Curve

Recombinant Human sFRP‑3 (Catalog # 192-SF) was serially diluted and captured by Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB1921) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human/Mouse sFRP‑3 Monoclonal Antibody (Catalog # MAB192) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for sFRP-3 Antibody (133931)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human sFRP-3 (Catalog # 192-SF)
Recombinant Mouse sFRP-3 (Catalog # 592-FR)
under non-reducing conditions only

Human/Mouse sFRP-3 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human/Mouse sFRP‑3 Biotinylated Antibody (Catalog # BAM192)
  • Standard: Recombinant Human sFRP-3 Protein (Catalog # 192-SF)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: sFRP-3

Secreted Frizzled Related Protein 3 (sFRP-3) was originally identified in bovine cartilage for its chondrogenic ability. Human, mouse, chick and Xenopus clones have also been isolated. SFRP-3 is often referred to as FRZB, other names include Fritz, Frzb1, and FRP-3. At the amino acid sequence level, sFRP-3 is highly conserved. The human protein shares 77% identity with Xenopus, 92% with mouse, and 94% with bovine proteins. Human sFRP-3 is strongly expressed in the developing appendicular skeleton, and cartilage of craniofacial bones. As determined by Northern blot of adult tissues, it is strongly detected in heart and placenta, as well as the brain, skeletal muscle, kidney and pancreas. The N-terminal portion of the protein shows 50% amino acid identity to the corresponding region of the Drosophila frizzled gene product, a receptor for Wg/Wnt signals. The similarity of sFRP-3 with frizzled proteins is restricted to the N-terminal cysteine-rich domain (CRD) that contains at least ten cysteine residues with highly conserved spacing between them. SFRP-3 was subsequently shown to be a soluble antagonist of Wnt signals. It lacks all transmembrane domains of frizzled proteins but retains the ability to bind Wnts. Ectopic expression of sFRP-3 mRNA has been shown to interfere with the induction of secondary axes in Xenopus embryos injected with Xwnt-8 mRNA.

References

  1. Hoang, et al. (1996) J. Biol. Chem. 271:26131.
  2. Leyns, et al. (1997) Cell 88:747.
  3. Wang, et al. (1997) Cell 88:757.
  4. Mayr, et al. (1997) Mech. Dev. 63:109.
  5. Rattner, et al. (1997) PNAS 94:2859.

Long Name

Secreted Frizzled Related Protein 3

Alternate Names

Fritz, FRZB, Frzb1, sFRP3

Entrez Gene IDs

2487 (Human); 20378 (Mouse)

Gene Symbol

FRZB

UniProt

AAB51298

Additional sFRP-3 Products

Product Documents for sFRP-3 Antibody (133931)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for sFRP-3 Antibody (133931)

For research use only

Related Research Areas

Wnt Family
Wnt Inhibitors

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