Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 628430
View all TORC2 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human TORC2
Lys453-Ser613
Accession # Q53ET0

Specificity

Detects human and mouse TORC2 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse TORC2 Antibody

Detection of Human and Mouse TORC2 antibody by Western Blot.

Detection of Human and Mouse TORC2 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, MDA-MB-453 human breast cancer cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.01 µg/mL of Mouse Anti-Human/Mouse TORC2 Monoclonal Antibody (Catalog # MAB6338) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for TORC2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse TORC2 Antibody

Application
Recommended Usage

Western Blot

0.01 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, U2OS human osteosarcoma cell line, MDA‑MB‑453 human breast cancer cell line, and NIH‑3T3 mouse embryonic fibroblast cell line

Reviewed Applications

Read 1 review rated 5 using MAB6338 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TORC2

TORC2, also known as CREB-regulated transcription co-activator 1 (crtc2), is an approximately 75 kDa protein that functions as a co‑activator for CREB1 in promoting transcription through both consensus and variant cAMP response element (CRE) sites. TORC2 activity is important in regulating the expression of genes involved in cellular energy metabolism. Its O-glycosylation triggers TORC2-dependent induction of hepatic gluconeogenic enzymes. It mediates hypothalamic glucose sensing and also the sensing of endoplasmic reticulum stress. In addition, TORC2 contributes to the transcription of HTLV-1 genes and the activation of latent EBV infections. Within aa 453‑613, human TORC2 shares 84% aa sequence identity with mouse TORC2.

Long Name

Transducer of Regulated CREB protein 1

Alternate Names

CRTC2

Entrez Gene IDs

200186 (Human); 74343 (Mouse); 310615 (Rat)

Gene Symbol

CRTC2

UniProt

Q53ET0 (Human)

Additional TORC2 Products

Product Documents for Human/Mouse TORC2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse TORC2 Antibody

For research use only

Citations for Human/Mouse TORC2 Antibody

Customer Reviews for Human/Mouse TORC2 Antibody (1)

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  • Human/Mouse TORC2 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: MDA-MB-231 human breast cancer cell line
    Species: Mouse
    Verified Customer | Posted 08/05/2025
    Human/Mouse TORC2 Antibody MAB6338

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