Human/Mouse Wnt-10a Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB34691G Datasheet / COA / SDS

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FAB34691G-100UG

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Human/Mouse Wnt-10a Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human, Mouse

Specificity

Detects mouse and human Wnt-10a in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse Wnt-1, -2, -2b, -3a, -4, -5a, -5b, recombinant human (rh) Wnt-6 or rhWnt-7a is observed.

Source

Monoclonal Rat IgG1 Clone # 342811

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant mouse Wnt-10a
Leu55-Glu95 and Gln278-Arg360
Accession # P70701

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Wnt-10a

Wnt-10a is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. Mouse Wnt-10a is synthesized as a 417 amino acid (aa) precursor that contains a 382 aa mature region. The mature region contains 24 cysteines and two potential N-linked glycosylation sites. Mature mouse Wnt-10a shares 96% and 99% aa sequence identity with mature human and rat Wnt-10a, respectively. Wnt-10a shares 64% aa sequence identity with Wnt-10b/12. Wnt-10a is produced by tumors and thymic epithelium.

Long Name

Wingless-type MMTV Integration Site Family Member 10A

Entrez Gene IDs

80326 (Human); 22409 (Mouse); 316527 (Rat)

Alternate Names

FLJ14301; protein Wnt-10a; SSPS; wingless-type MMTV integration site family, member 10A; Wnt10a; Wnt-10a