ZBTB7A/Pokemon Antibody (466407)

R&D Systems | Catalog # MAB3496

R&D Systems

Key Product Details

Species Reactivity

Human, Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 466407
View all ZBTB7A/Pokemon Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human ZBTB7A/Pokemon
Met349-Val476
Accession # O95365

Specificity

Detects human and mouse ZBTB7A/Pokemon in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for ZBTB7A/Pokemon Antibody (466407)

Detection of Human and Mouse ZBTB7A/Pokemon antibody by Western Blot.

Detection of Human and Mouse ZBTB7A/Pokemon by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse ZBTB7A/Pokemon Monoclonal Antibody (Catalog # MAB3496) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ZBTB7A/Pokemon at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 10.

Applications for ZBTB7A/Pokemon Antibody (466407)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF‑7 human breast cancer cell line, HepG2 human hepatocellular carcinoma cell line, and NIH‑3T3 mouse embryonic fibroblast cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ZBTB7A/Pokemon

ZBTB7A (zinc finger and BTB domain-containing 7A), also known as Pokemon, FBI-1 in human, LRF in mouse, and OCZF in rat, is a widely expressed nuclear protein that is overexpressed in many human cancers. It is a transcriptional repressor of ARF, c-fos, c-myc, Rb and others, including many extracellular matrix genes. These targets play important roles, both in normal differentiation and oncogenesis. Human ZBTB7A/Pokemon is a 584 amino acid (aa) protein with a BTB-POZ domain (aa 24‑131) and four zinc finger repeats (aa 382‑484). Human ZBTB7A/Pokemon shares 98% aa identity with mouse and rat within aa 349‑476.

Long Name

Zinc Finger and BTB Domain-Containing Protein 7A

Alternate Names

FBI-1, LRF, Pokemon, TIP21

Entrez Gene IDs

51341 (Human); 16969 (Mouse); 117107 (Rat)

Gene Symbol

ZBTB7A

UniProt

O95365

Additional ZBTB7A/Pokemon Products

Product Documents for ZBTB7A/Pokemon Antibody (466407)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for ZBTB7A/Pokemon Antibody (466407)

For research use only

Citations for ZBTB7A/Pokemon Antibody (466407)

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