Human NFATC2 Antibody (639402)

Detection of Human NFATC2 by Western Blot.

Western blot shows lysates of BJAB human Burkitt's lymphoma cell line, Jurkat human acute T cell leukemia cell line, and MOLT-4 human acute lymphoblastic leukemia cell line. PVDF Membrane was probed with 0.5 µg/mL of Human NFATC2 Monoclonal Antibody (Catalog # MAB6499) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NFATC2 at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of drembtgr66hzstlf4xjk.jpg by Western Blot

Regulation of NFATC2 on glioma cell proliferation. NFATC2 was overexpressed in U251 cells using a lentiviral expression vector, and proliferation was assessed 72 h post‐transfection. (A) NFATC2 mRNA levels in U251 cells after NFATC2 overexpression. **p < 0.01. (B) Western blotting for NFATC2 protein in U251 cells after NFATC2 overexpression. (C) U251 cell images after NFATC2 overexpression (OE). (D) Cell viability measured by the MTT assay at different culture times and fold change for U251 cells after NFATC2 overexpression. (E) Observation of U251 cell colonies after 23 days of NFATC2 overexpression. (F) U251 cells were collected 5 days after NFATC2 overexpression and the DNA content was determined by flow cytometry with propidium iodide (PI) staining. The percentage of cells in the G1, S and G2/M phases was measured. **p < 0.01. (G) Cells were subjected to flow cytometry with Annexin V‐APC staining. The percentage of apoptotic cells was measured. **p < 0.01. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41578162), licensed under a CC-BY license. Not internally tested by R&D Systems.

Detection of NFATC2 by Western Blot

Analysis of MRPS16 target genes. (A) MRPS16 was overexpressed in glioblastoma U251 cells. Three days later, U251 cell images were taken. (B) Detection of 96 downstream gene expression levels by RT‐PCR after MRPS16 knockdown. (C) The expression level of the 15 most differentially expressed genes. (D) Regulation interaction map of the 15 differentially expressed genes. Red indicates up‐regulation and green down‐regulation. Solid arrows indicate definite regulatory relationships and dashed arrows indicate predicted regulatory relationships. (E) Western blotting for NFATC2 protein in U251 cells under MRPS16 knockdown. (F) NFATC2 expression levels in normal and glioma tissues from the TCGA database. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/41578162), licensed under a CC-BY license. Not internally tested by R&D Systems.