Human NG2/MCSP Antibody

Catalog #: AF2585
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2585
AF2585-SP

Save 15% on Select RUO Reagents. See Details.

Detection of Human NG2/MCSP by Western Blot.

2 Images

All NG2/MCSP Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human NG2/MCSP Antibody Summary

Species Reactivity

Human

Specificity

Detects human NG2/MCSP in direct ELISAs and Western blots. In Western blots, approximately 30% cross-reactivity with recombinant mouse CSPG4 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant human NG2/MCSP
Ser1583-Ser2224
Accession # Q6UVK1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Knockout Validated

NG2/MCSP is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in NG2/MCSP knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human NG2/MCSP by Western Blot. Western blot shows lysates of A375 human melanoma cell line and SK-Mel-28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human NG2/MCSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2585) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human NG2/MCSP Antibody Specificity by Using Knockout Cell Line.

View Larger

Western Blot Shows Human NG2/MCSP Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and NG2/MCSP knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human NG2/MCSP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2585) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for NG2/MCSP at approximately 300 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: NG2/MCSP

NG2 (neuron/glia-type 2 antigen; also MCSP/melanoma chondroitin sulfate proteoglycan) is a 250‑500 kDa type I integral membrane proteoglycan found on synantocytes (NG2+ glia), vascular pericytes, chondroblasts, macrophages and melanoma cells. It binds multiple components of the ECM, and serves as a ligand for alpha 3 beta 1 and alpha 4 beta 1 integrins. Mature human NG2 is 2293 amino acids (aa) in length. It contains a large 2195 aa extracellular region (aa 30‑2224) and a short 79 aa cytoplasmic tail. NG2 is variably glycanated. Without proteoglycan, NG2 is expressed as a 250 kDa glycoprotein. Juxtamembrane proteolysis generates a soluble ECD that noncovalently associates with the transmembrane fragment. Over aa 1583‑2224, human NG2 is 81% aa identical to both mouse and canine NG2.

Long Name

Chondroitin Sulfate Proteoglycan NG2/Melanoma Associated Chondroitin Sulfate Proteoglycan

Entrez Gene IDs

1464 (Human); 121021 (Mouse); 81651 (Rat)

Alternate Names

AN2; chondroitin sulfate proteoglycan 4 (melanoma-associated); chondroitin sulfate proteoglycan 4; CSPG4; EC 3.6.3; HMW-MAA; MCSP; MCSPChondroitin sulfate proteoglycan NG2; MCSPG; MCSPGMelanoma chondroitin sulfate proteoglycan; MEL-CSPG; MSK16Melanoma-associated chondroitin sulfate proteoglycan; NG2; NG2EC 2.7.8

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human NG2/MCSP Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Proteomic profile of melanoma cell‐derived small extracellular vesicles in patients’ plasma: a potential correlate of melanoma progression
Authors: Monika Pietrowska, Aneta Zebrowska, Marta Gawin, Lukasz Marczak, Priyanka Sharma, Sujan Mondal et al.
Journal of Extracellular Vesicles
IGFBP7, a novel tumor stroma marker, with growth-promoting effects in colon cancer through a paracrine tumor-stroma interaction.
Authors: Rupp C, Scherzer M, Rudisch A, Unger C, Haslinger C, Schweifer N, Artaker M, Nivarthi H, Moriggl R, Hengstschlager M, Kerjaschki D, Sommergruber W, Dolznig H, Garin-Chesa P
Oncogene, 2014-03-17;34(7):815-25.
Species: Human
Sample Types: Whole Tissue
Applications: IHC, IHC-Fr