Human NOV/CCN3 Antibody
Human NOV/CCN3 Antibody Summary
Accession # P48745
under non-reducing conditions only
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
NOV, also called CCN3, is one of six CCN (CYR61/CTGF/NOV) secreted proteins which share a common multimodular organization (1 - 4). NOV/CCN3 contains an N-terminal IGFBP domain that appears to be non-functional and a vWF type C and thrombospondin type I domain which mediate oligomerization and matrix interactions, respectively (1, 2). The C-terminal cysteine knot domain interacts with several partners, including the matrix protein fibulin 1C (5), Notch-1 (6), and CCN2, with which it may heterodimerize (2). NOV/CCN3 also interacts with the gap junction protein Connexin43 and mediates suppression of proliferation (7). It also binds the calcium binding protein S100A4 and promotes calcium channel activation (8). The 357 amino acid (aa), 44 kDa human NOV/CCN3 shares 80% aa identity with mouse, rat and dog NOV/CCN3, and 78% aa identity with cow NOV/CCN3. NOV/CCN3 also shows 38 - 50% aa identity with other family members including WISP proteins, except for WISP-2/CCN5 which lacks the cysteine knot (1). NOV/CCN3 is widely expressed developmentally, especially in muscle, endothelium, nervous system, adrenal cortex and chondrocytes (1 - 4). In transformed cells, a 32 kDa N-terminally truncated form lacks the signal sequence is localized to the nucleus. Truncation allows a C-terminal nuclear localization sequence to be active (9). Nuclear NOV/CCN3 acts as a transcriptional repressor but promotes proliferation, presumably by interfering with growth control (9). Full length NOV/CCN3 is a secreted matricellular protein which inhibits cell growth. Interaction of NOV/CCN3 with integrins alpha v beta 3 and alpha 5 beta 1 mediates endothelial cell adhesion, chemotaxis, and promotes angiogenesis (10, 11). Over-expression of NOV/CCN3 downregulates myogenic genes such as MyoD (12).
- Perbal, B. (2004) Lancet 363:62.
- Perbal, B. (2006) Cell Commun. Signal. 4:3.
- Martinerie, C. et al. (1994) Oncogene 9:2729.
- Snaith, M.R. et al. (1996) Genomics 38:425.
- Perbal, B. et al. (1999) Proc. Natl. Acad. Sci. USA 96:869.
- Sakamoto, K. et al. (2002) J. Biol. Chem. 277:29399.
- Fu, C.T. et al. (2004) J. Biol. Chem. 279:36943.
- Li, C.L. et al. (2002) Mol. Pathol. 55:250.
- Planque, N. et al. (2006) J. Cell. Biochem. 99:105.
- Lin, C.G. et al. (2003) J. Biol. Chem. 278:24200.
- Lin, C.G. et al. (2003) J. Biol. Chem. 280:8229.
- Calhabeu, F. et al. (2006) Exp. Cell. Res. 312:1876.
Citations for Human NOV/CCN3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 2
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Anti-CTGF single-chain variable fragment dimers inhibit human airway smooth muscle (ASM) cell proliferation by down-regulating p-Akt and p-mTOR levels.
Authors: Gao W, Cai L, Xu X, Fan J, Xue X, Yan X, Qu Q, Wang X, Zhang C, Wu G
PLoS ONE, 2014-12-05;9(12):e113980.
Sample Types: Recombinant Protein
Applications: ELISA Development
CCN3 (NOV) is a negative regulator of CCN2 (CTGF) and a novel endogenous inhibitor of the fibrotic pathway in an in vitro model of renal disease.
Authors: Riser BL, Najmabadi F, Perbal B, Peterson DR, Rambow JA, Riser ML, Sukowski E, Yeger H, Riser SC
Am. J. Pathol., 2009-04-09;174(5):1725-34.
Sample Types: Cell Culture Supernates, Cell Lysates
Applications: ELISA Development, Western Blot
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