Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

Immunocytochemistry, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 36526
View all NT-4 Primary Antibodies »

Product Specifications

Immunogen

E. coli derived recombinant human NT-4

Specificity

Detects human NT-4 in direct ELISAs and Western blots. In Western blots, this antibody shows less than 1% cross‑reactivity with recombinant human (rh) NT-3 and rhBDNF.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human NT-4 Antibody (36526)

Human NT-4 ELISA Standard Curve

Human NT-4 ELISA Standard Curve

Recombinant Human NT‑4 (Catalog # 268-N4) was serially diluted and captured by Mouse Anti-Human NT‑4 Monoclonal Antibody (Catalog # MAB268) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human NT‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-268-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human NT-4 Antibody (36526)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human NT‑4 (Catalog # 268-N4)
under non-reducing conditions only

Human NT-4 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human NT‑4 Biotinylated Antibody (Catalog # BAF268)
  • Standard: Recombinant Human NT-4 Protein (Catalog # 268-N4)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: NT-4

NT-4 was originally identified as a mammalian homolog of the Xenopus neurotrophin, NT-4. Along with NGF, BDNF, and NT-3, it is a member of the NGF family of neurotrophic factors that are required for the differentiation and survival of specific neuronal subpopulations in both the central and the peripheral nervous systems. NT-4 functions through interactions with the TrkB receptor tyrosine kinase. Because mammalian NT-4 is much more divergent from its amphibian functional counterpart (Xenopus NT-4) than other family members, it is sometimes designated NT-5 or NT-4/5.

Long Name

Neurotrophin 4

Alternate Names

NT-5, NT4, NTF4, NTF5

Entrez Gene IDs

4909 (Human)

Gene Symbol

NTF4

Additional NT-4 Products

Product Documents for Human NT-4 Antibody (36526)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human NT-4 Antibody (36526)

For research use only

Citations for Human NT-4 Antibody (36526)

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