Human Osterix/Sp7 MAb (Clone 764746)

Catalog #: FAB75471X Datasheet / COA / SDS

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FAB75471X-100UG

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All Osterix/Sp7 Antibodies

Product Details

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Osterix/Sp7 MAb (Clone 764746) Summary

Species Reactivity

Human

Specificity

Detects human Osterix/Sp7 in direct ELISAs.

Source

Monoclonal Mouse IgG2b Clone # 764746

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human Osterix/Sp7
Met19-Leu288
Accession # Q8TDD2

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: Osterix/Sp7

Osterix, also known as Sp7, is an approximately 45 kDa transcription factor that is required for osteogenesis and bone homeostasis. Osterix cooperates with BMP-6 in the differentiation of osteoblasts from mesenchymal stem cells by regulating the transcription of several genes involved in osteoblast differentiation and function (i.e. SATB2, Collagen V, and SOST). The transcription of Osterix is induced by BMP-2, IGF-I, and parathyroid hormone, while its activity is regulated by Akt and p38 MAPK-mediated phoshorylation. Osterix contains a transactivation domain (aa 141-210) and three zinc finger domains (aa 294-318, aa 324-348, and aa 354-376). Alternative splicing generates a short isoform that lacks the N-terminal 18 amino acids. Within aa 19-288, human Osterix shares 94% aa sequence identity with mouse and rat Osterix.

Long Name

Sp7 Transcription Factor

Entrez Gene IDs

121340 (Human); 170574 (Mouse); 300260 (Rat)

Alternate Names

MGC126598; Osterix; OSX; OSXosterix; Sp7 transcription factor; Sp7; transcription factor Sp7; Zinc finger protein osterix