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Human P-Selectin/CD62P ELISA Kit

Catalog #: BBE6
29 Citations 2 Reviews Datasheet / COA / SDS
Newer Version Available: DPSE00
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Human P-Selectin/CD62P Standard Curve
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Product Details
Procedure
Citations (29)
FAQs
Reviews (2)
Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human P-Selectin/CD62P ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
1.25 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 uL), Serum (10 uL), EDTA Plasma (10 uL), Heparin Plasma (10 uL), Citrate Plasma (10 uL)
Sensitivity
0.5 ng/mL
Assay Range
0.8 - 46 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
Specificity
Natural and recombinant human sP-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.Cross-species reactivity not tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Human sP-Selectin Immunoassay is a 1.25 hour solid phase ELISA that measures sP-Selectin in cell culture supernates, serum, and plasma. It contains recombinant human sP-Selectin and antibodies raised against the recombinant factor. It has been shown to accurately quantitate the recombinant sP-Selectin. Results obtained using natural human sP-Selectin showed linear curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural human sP-Selectin.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 10 10 10 18 18 18
Mean (pg/mL) 84 430 668 94 451 730
Standard Deviation 4.28 21.1 37.4 9.31 39.7 57.7
CV% 5.1 4.9 5.6 9.9 8.8 7.9

Recovery

The recovery of sP-Selectin spiked to three different levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=5) 105 98-115
Citrate Plasma (n=7) 98 82-111
EDTA Plasma (n=7) 94 78-107
Heparin Plasma (n=7) 100 77-114
Serum (n=7) 101 84-119

Linearity

To assess the linearity of the assay, natural or spiked samples of each type were diluted in Sample Diluent and assayed in three batches of kits.
Human P-Selectin/CD62P ELISA Linearity

Scientific Data

N/A Human P-Selectin/CD62P Standard Curve View Larger

Human P-Selectin/CD62P Standard Curve

Product Datasheets

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Product Datasheet
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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: P-Selectin/CD62P

The Selectin family is comprised of three members, E-Selectin, L-Selectin, and P-Selectin. E-Selectin [endothelial leukocyte adhesion molecule-1 (ELAM-1), CD62E] is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha. The human and rat proteins share approximately 67% amino acid sequence identity. The mouse and rat proteins share approximately 78% amino acid sequence identity. L-Selectin (Leukocyte Selectin, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, lymph node homing receptor, CD62L) is expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. Human and mouse L-Selectin share 76% amino acid sequence identity. Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P) is expressed by activated platelets and endothelial cells. The extracellular domains of human and mouse P-Selectin share approximately 73% amino acid sequence identity.

Entrez Gene IDs:
6403 (Human); 20344 (Mouse)
Alternate Names:
CD62P antigen; CD62P; FLJ45155; GMP140; GRMP; PADGEM; PADGEMantigen CD62); PSEL; P-Selectin; selectin P (granule membrane protein 140kDa, antigen CD62); SELP

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, samples, and Control as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Standard, Control, or sample
  3.   Add 100 µL of Standard, Control, or sample to each well.
    4. 100 µL Conjugate
  4.   Add 100 µL of Conjugate to each well. Tap the plate gently to mix. Cover with a plate sealer, and incubate at room temperature for 1 hour.
  5.   Aspirate or decant each well and wash, repeating the process twice for a total of 3 washes.
    6. 100 µL Substrate
  6.   Add 100 µL of Substrate to each well. Cover with a new plate sealer, and incubate at room temperature for 15 minutes.
    7. 100 µL Stop Solution
  7.   Add 100 µL Stop Solution to each well. The Stop Solution should be added to the wells in the same order as the Substrate. Read at 450 nm within 30 minutes. Set wavelength correction to 620 nm or 650 nm.

Citations for Human P-Selectin/CD62P ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

29 Citations: Showing 1 - 10
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  1. Biomarkers for the risk of thrombosis in pancreatic adenocarcinoma are related to cancer process
    Authors: D Faille, MC Bourrienne, E de Raucour, L de Chaisem, V Granger, R Lacroix, L Panicot-Du, P Hammel, P Lévy, P Ruszniewsk, N Ajzenberg, V Rebours
    Oncotarget, 2018-05-29;9(41):26453-26465.
    Species: Human
    Sample Types: Plasma
  2. Acute effect of different concentrations of cayenne pepper cataplasm on sensory-motor functions and serum levels of inflammation-related biomarkers in healthy subjects
    Authors: N Sarabon, S Löfler, J Cvecka, W Hübl, S Zampieri
    Eur J Transl Myol, 2018-03-01;28(1):7333.
    Species: Human
    Sample Types: Serum
  3. Optimized plasma preparation is essential to monitor platelet-stored molecules in humans
    Authors: M Mussbacher, WC Schrottmai, M Salzmann, C Brostjan, JA Schmid, P Starlinger, A Assinger
    PLoS ONE, 2017-12-08;12(12):e0188921.
    Species: Human
    Sample Types: Plasma
  4. Platelet Carbonic Anhydrase II, a Forgotten Enzyme, May Be Responsible for Aspirin Resistance
    Authors: M Jakubowski, J D?bski, E Szahidewic, A Turek-Jaku, J Gawry?, K Gawry?, R Skomro, A Derkacz, A Doroszko
    Oxid Med Cell Longev, 2017-09-27;2017(0):3132063.
    Species: Human
    Sample Types: Plasma
  5. Dietary and genetic influences on hemostasis in a Yup'ik Alaska Native population
    Authors: NT Au, M Reyes, BB Boyer, SE Hopkins, J Black, D O'Brien, AE Fohner, J Yracheta, T Thornton, MA Austin, W Burke, KE Thummel, AE Rettie
    PLoS ONE, 2017-04-04;12(4):e0173616.
    Species: Human
    Sample Types: Cell Culture Supernates
  6. Association between obstructive sleep apnea severity and endothelial dysfunction in patients with type 2 diabetes
    Authors: V Bironneau, F Goupil, PH Ducluzeau, M Le Vaillan, P Abraham, S Henni, S Dubois, A Paris, P Priou, N Meslier, C Sanguin, W Trzépizur, R Andriantsi, MC Martinez, F Gagnadoux
    Cardiovasc Diabetol, 2017-03-21;16(1):39.
    Species: Human
    Sample Types: Serum
  7. Neutrophil-derived microparticles are released into the coronary circulation following percutaneous coronary intervention in acute coronary syndromes
    Biosci. Rep, 2017-01-17;0(0):.
    Species: Human
    Sample Types: Plasma
  8. Cardiovascular diseases-related GNB3 C825T polymorphism has a significant sex-specific effect on serum soluble E-selectin levels
    Authors: Kokoè Mélinda Gbadoe
    J Inflamm (Lond), 2016-12-09;13(0):39.
    Species: Human
    Sample Types: Serum
  9. Effects of radiofrequency catheter ablation of atrial fibrillation on soluble P-selectin, von Willebrand factor and IL-6 in the peripheral and cardiac circulation.
    Authors: Kornej J, Dinov B, Blann A, Rolf S, Arya A, Schmidl J, Husser D, Hindricks G, Bollmann A, Lip G
    PLoS ONE, 2014-11-12;9(11):e111760.
    Species: Human
    Sample Types: Plasma
  10. Immune-inflammatory markers and arterial stiffness indexes in subjects with acute ischemic stroke with and without metabolic syndrome.
    Authors: Tuttolomondo A, Pecoraro R, Di Raimondo D, Di Sciacca R, Canino B, Arnao V, Butta C, Della Corte V, Maida C, Licata G, Pinto A
    Diabetol Metab Syndr, 2014-02-27;6(1):28.
    Species: Human
    Sample Types: Plasma
  11. Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer.
    Authors: Tavoosidana G, Ronquist G, Darmanis S, Yan J, Carlsson L, Wu D, Conze T, Ek P, Semjonow A, Eltze E, Larsson A, Landegren UD, Kamali-Moghaddam M
    Proc. Natl. Acad. Sci. U.S.A., 2011-05-09;108(21):8809-14.
    Species: Human
    Sample Types: Platelet Poor Plasma
  12. Persistently raised plasma levels of platelet-derived inflammatory mediators in HIV-infected patients during highly active anti-retroviral therapy.
    Authors: Landro L, Ueland T, Otterdal K, Froland SS, Aukrust P
    J. Thromb. Haemost., 2011-05-01;9(5):1075-7.
    Species: Human
    Sample Types: Platelet Poor Plasma
  13. Thrombospondin-1: a unique marker to identify in vitro platelet activation when monitoring in vivo processes.
    Authors: Starlinger P, Moll HP
    J. Thromb. Haemost., 2010-05-12;0(0):.
    Species: Human
    Sample Types: Serum
  14. Endothelial activation, inflammation and premature atherosclerosis in children with familial dyslipidemia.
    Authors: Guardamagna O, Abello F, Saracco P
    Atherosclerosis, 2009-06-11;207(2):471-5.
    Species: Human
    Sample Types: Serum
  15. HIV increases markers of cardiovascular risk: results from a randomized, treatment interruption trial.
    Authors: Calmy A, Gayet-Ageron A, Montecucco F, Nguyen A, Mach F, Burger F, Ubolyam S, Carr A, Ruxungtham K, Hirschel B, Ananworanich J
    AIDS, 2009-05-15;23(8):929-39.
    Species: Human
    Sample Types: Plasma
  16. Immuno-inflammatory predictors of stroke at follow-up in patients with chronic non-valvular atrial fibrillation (NVAF).
    Authors: Pinto A, Tuttolomondo A, Casuccio A, Di Raimondo D, Di Sciacca R, Arnao V, Licata G
    Clin. Sci., 2009-05-01;116(10):781-9.
    Species: Human
    Sample Types: Plasma
  17. Pharmacodynamics and Pharmacokinetics of the Novel Thrombopoietin Mimetic Peptide RWJ-800088 in Humans.
    Authors: Liem-Moolenaar M, Cerneus D, Molloy C, End D, Brown K, de Kam M, Cohen A, van Hensbergen Y, Burggraaf J
    2008-10-01;84(0):481.
    Species: Human
    Sample Types: Plasma
  18. Cross-sectional relations of multiple inflammatory biomarkers to peripheral arterial disease: The Framingham Offspring Study.
    Authors: Murabito JM, Keyes MJ, Guo CY, Keaney JF, Vasan RS, D'Agostino RB, Benjamin EJ
    Atherosclerosis, 2008-07-09;203(2):509-14.
    Species: Human
    Sample Types: Plasma
  19. Immuno-inflammatory and thrombotic/fibrinolytic variables associated with acute ischemic stroke diagnosis.
    Authors: Tuttolomondo A, Pinto A, Corrao S, Di Raimondo D, Fernandez P, Di Sciacca R, Arnao V, Licata G
    Atherosclerosis, 2008-07-09;203(2):503-8.
    Species: Human
    Sample Types: Plasma
  20. Pulmonary arterial hypertension and left-sided heart disease in sickle cell disease: clinical characteristics and association with soluble adhesion molecule expression.
    Authors: Klings ES, Anton Bland D, Rosenman D, Princeton S, Odhiambo A, Li G, Bernard SA, Steinberg MH, Farber HW
    Am. J. Hematol., 2008-07-01;83(7):547-53.
    Species: Human
    Sample Types: Plasma
  21. Soluble P-selectin, SELP polymorphisms, and atherosclerotic risk in European-American and African-AFrican young adults: the Coronary Artery Risk Development in Young Adults (CARDIA) Study.
    Authors: Reiner AP, Carlson CS, Thyagarajan B, Rieder MJ, Polak JF, Siscovick DS, Nickerson DA, Jacobs DR, Gross MD
    Arterioscler. Thromb. Vasc. Biol., 2008-06-05;28(8):1549-55.
    Species: Human
    Sample Types: Plasma
  22. Fish oil in combination with high or low intakes of linoleic acid lowers plasma triacylglycerols but does not affect other cardiovascular risk markers in healthy men.
    Authors: Damsgaard CT, Frokiaer H, Andersen AD, Lauritzen L
    J. Nutr., 2008-06-01;138(6):1061-6.
    Species: Human
    Sample Types: Plasma
  23. Elevated urinary VCAM-1, P-selectin, soluble TNF receptor-1, and CXC chemokine ligand 16 in multiple murine lupus strains and human lupus nephritis.
    Authors: Wu T, Xie C, Wang HW, Zhou XJ, Schwartz N, Calixto S, Mackay M, Aranow C, Putterman C, Mohan C
    J. Immunol., 2007-11-15;179(10):7166-75.
    Species: Human
    Sample Types: Urine
  24. Pleiotrophic and anti-inflammatory effects of pioglitazone precede the metabolic activity in type 2 diabetic patients with coronary artery disease.
    Authors: Forst T, Karagiannis E, Lubben G, Hohberg C, Schondorf T, Dikta G, Drexler M, Morcos M, Danschel W, Borchert M, Pfutzner A
    Atherosclerosis, 2007-06-22;197(1):311-7.
    Species: Human
    Sample Types: Serum
  25. Significant decrease in angiopoietin-1 and angiopoietin-2 after radical prostatectomy in prostate cancer patients.
    Authors: Caine GJ, Ryan P, Lip GY, Blann AD
    Cancer Lett., 2007-01-19;251(2):296-301.
    Species: Human
    Sample Types: Plasma
  26. Interferon-beta and adhesion molecules (E-selectin and s-intracellular adhesion molecule-1) are detected in sera from patients with retinal vasculitis and are induced in retinal vascular endothelial cells by Toll-like receptor 3 signalling.
    Authors: Lee MT, Hooper LC, Kump L, Hayashi K, Nussenblatt R, Hooks JJ, Detrick B
    Clin. Exp. Immunol., 2007-01-01;147(1):71-80.
    Species: Human
    Sample Types: Serum
  27. No difference in the effects of clopidogrel and aspirin on inflammatory markers in patients with coronary heart disease.
    Authors: Solheim S, Pettersen AA, Arnesen H, Seljeflot I
    Thromb. Haemost., 2006-11-01;96(5):660-4.
    Species: Human
    Sample Types: Plasma
  28. Changes in plasma vascular endothelial growth factor, angiopoietins, and their receptors following surgery for breast cancer.
    Authors: Caine GJ, Stonelake PS, Lip GY, Blann AD
    Cancer Lett., 2006-08-07;248(1):131-6.
    Species: Human
    Sample Types: Plasma
  29. Association between enhanced soluble CD40 ligand and prothrombotic state in inflammatory bowel disease.
    Authors: Koutroubakis IE, Theodoropoulou A, Xidakis C, Sfiridaki A, Notas G, Kolios G, Kouroumalis EA
    Eur J Gastroenterol Hepatol, 2004-11-01;16(11):1147-52.
    Species: Human
    Sample Types: Platelet Poor Plasma

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Human P-Selectin/CD62P ELISA Kit
By Anna Picca on 06/19/2019
Sample Tested: Serum
Human P-Selectin/CD62P ELISA Kit BBE6
Human P-Selectin/CD62P ELISA Kit
By Ann Cullinane on 07/17/2017
Sample Tested: Citrate Plasma