P-Selectin, also known as CD62P, GMP-140, PADGEM, and LECAM-3, is a transmembrane adhesion protein that is upregulated on activated platelets and vascular endothelial cells. It binds to hematopoietic cell PSGL-1/CD162 with highly clustered O-linked sialyl Lewis-x (sLex ) tetrasaccharides and sulfated tyrosine, as well as to heparin sulfate, Versican, and CD42b/GPIb alpha. P-Selectin mediates the initial interaction of circulating leukocytes with activated endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. It is therefore critical for leukocyte extravasation at sites of inflammation and leukocyte involvement in thrombus formation at sites of vascular injury.
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Human P-Selectin/CD62P Quantikine ELISA Kit
R&D Systems | Catalog # DPSE00
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Key Product Details
Assay Length
1.25 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (10 µL), Serum (10 µL), EDTA Plasma (10 µL), Heparin Plasma (10 µL), Citrate Plasma (10 µL)
Sensitivity
0.121 ng/mL
Assay Range
0.781-50 ng/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma)
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Product Summary for Human P-Selectin/CD62P Quantikine ELISA Kit
The Quantikine® Human P-Selectin/CD62P Immunoassay is a 1.25 hour solid-phase ELISA designed to measure human P-Selectin in cell culture supernates, serum, and plasma. It contains recombinant human P-Selectin and antibodies raised against the recombinant factor. Results obtained using natural human P-Selectin showed linear curves that were parallel to the standard curves obtained using the Quantikine® kit standards. These results indicate that this kit can be used to determine relative mass values for natural human P-Selectin.
Product Specifications
Measurement
Quantitative ELISA
Detection Method
Colorimetric - 450nm (TMB)
Conjugate
HRP
Species
Human
Specificity
Rrecombinant and natural human P-Selectin
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
Sample Values
Serum/Plasma - Samples from apparently healthy volunteers were evaluated for the presence
of human P-Selectin in this assay. No medical histories were available for the donors used in
this study.
ND=Non-detectable
| Sample Type | Mean (ng/mL) | Range (ng/mL) | Standard Deviation (ng/mL) |
| Serum (n=33) | 82.6 | 43.5-119 | 20.0 |
| EDTA plasma (n=33) | 35.3 | 18.3-57.4 | 8.78 |
| Heparin plasma (n=33) | 33.7 | 18.6-50.6 | 7.40 |
| Sample Type | Mean of Detectable (ng/mL) | % Detectable | Range (ng/mL) |
| Citrate plasma (n=33) | 25.8 | 97 | ND-37.9 |
精度
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in twenty separate assays to assess inter-assay precision. Assays were performed by at least three technicians.
Cell Culture Supernates, Citrate Plasma, EDTA Plasma, Heparin Plasma, Serum
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 6.54 | 19.3 | 25.6 | 6.51 | 19.9 | 30.5 |
| Standard Deviation | 0.442 | 1.47 | 2.29 | 0.484 | 1.57 | 3.64 |
| CV% | 6.8 | 7.6 | 8.9 | 7.4 | 7.9 | 11.9 |
Recovery for Human P-Selectin/CD62P Quantikine ELISA Kit
The recovery of Human P-Selectin spiked to three different levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 97 | 88-107 |
| Citrate Plasma (n=4) | 92 | 81-111 |
| EDTA Plasma (n=4) | 92 | 77-103 |
| Heparin Plasma (n=4) | 95 | 78-112 |
| Serum (n=4) | 93 | 77-104 |
Linearity
To assess the linearity of the assay, samples spiked with high concentrations of human P-Selectin were diluted with sample diluent to produce samples with values within the dynamic range of the assay.
Scientific Data Images for Human P-Selectin/CD62P Quantikine ELISA Kit
Human P-Selectin ELISA Standard Curve
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: P-Selectin/CD62P
Alternate Names
CD62P, GMP140, GRMP, PADGEM, PSEL, SELP
Gene Symbol
SELP
Additional P-Selectin/CD62P Products
Product Documents for Human P-Selectin/CD62P Quantikine ELISA Kit
Product Specific Notices for Human P-Selectin/CD62P Quantikine ELISA Kit
For research use only
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.Citations for Human P-Selectin/CD62P Quantikine ELISA Kit
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Sample Tested: Citrate PlasmaVerified Customer | Posted 10/12/2021We diluted the plasma 1:5
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