Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 108128
View all PDGF-B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127

Specificity

Detects the human PDGF-B subunit in direct ELISAs and Western blots. In Western blots, shows 50-100% cross-reactivity with recombinant human (rh) PDGF‑AB and rrPDGF-BB but no cross-reactivity with rhCTGF, rhPlGF, rhVEGF‑B186, rhVEGF‑C, or rhVEGF‑D.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Human PDGF‑B Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human PDGF-B (Catalog # 220-BB)
under non-reducing conditions only

Human PDGF-AB Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human PDGF-AA/AB Biotinylated Antibody (Catalog # BAF221)
  • Standard: Recombinant Human PDGF-AB Protein, CF (Catalog # 222-AB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB1739 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-B

Four PDGF family members, A, B, C and D, have been identified. PDGF-B can exist as a disulfide-linked homodimer (PDGF-BB) or heterodimer (PDGF-AB). PDGF-B binds and activates PDGF R alpha and PDGF R beta.

Long Name

Platelet-derived Growth Factor B

Alternate Names

C-Sis, IBGC5, PDGF-2, PDGF2, PDGFB, SIS, SSV

Entrez Gene IDs

5155 (Human); 24628 (Rat); 100070283 (Equine)

Gene Symbol

PDGFB

UniProt

P01127

Additional PDGF-B Products

Product Documents for Human PDGF‑B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PDGF‑B Antibody

For research use only

Related Research Areas

PDGF Family

Citations for Human PDGF‑B Antibody

Customer Reviews for Human PDGF‑B Antibody (1)

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1 Customer Rating
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  • Human PDGF-B Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/19/2019
    Human PDGF‑B Antibody MAB1739

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