Human PDGF-B Antibody

Name: Human PDGF-B Antibody
Brand: R&D Systems
SKU: MAB1739-100
Rating: 5 (1 reviews)

Catalog #: MAB1739 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1739-SP
MAB1739-500
MAB1739-100

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF-B Antibody Summary

Species Reactivity

Human

Specificity

Detects the human PDGF-B subunit in direct ELISAs and Western blots. In Western blots, shows 50-100% cross-reactivity with recombinant human (rh) PDGF‑AB and rrPDGF-BB but no cross-reactivity with rhCTGF, rhPlGF, rhVEGF‑B₁₈₆, rhVEGF‑C, or rhVEGF‑D.

Source

Monoclonal Mouse IgG₁ Clone # 108128

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human PDGF-B (Catalog # 220-BB)
under non-reducing conditions only

Human PDGF-AB Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human PDGF-AA/AB Biotinylated Antibody (Catalog # BAF221)

Standard: Recombinant Human PDGF-AB Protein, CF (Catalog # 222-AB)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-B

Four PDGF family members, A, B, C and D, have been identified. PDGF-B can exist as a disulfide-linked homodimer (PDGF-BB) or heterodimer (PDGF-AB). PDGF-B binds and activates PDGF R alpha and PDGF R beta.

Long Name

Platelet-derived Growth Factor B

Entrez Gene IDs

5155 (Human); 24628 (Rat); 100070283 (Equine)

Alternate Names

B chain; Becaplermin; C-Sis; FLJ12858; IBGC5; PDGF subunit B; PDGF2; PDGF-2; PDGFB; PDGF-B; platelet-derived growth factor 2; platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis)oncogene homolog); Platelet-derived growth factor beta polypeptide; platelet-derived growth factor subunit B; platelet-derived growth factor, B chain; Platelet-derived growth factor, beta polypeptide (oncogene SIS); Proto-oncogene c-Sis; SIS; SSV