Human PDGF-B Antibody (108128)

R&D Systems | Catalog # MAB1739

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair)

Cited:

ELISA Capture

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 108128
View all PDGF-B Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human PDGF‑BB
Ser82-Thr190
Accession # P01127

Specificity

Detects the human PDGF-B subunit in direct ELISAs and Western blots. In Western blots, shows 50-100% cross-reactivity with recombinant human (rh) PDGF‑AB and rrPDGF-BB but no cross-reactivity with rhCTGF, rhPlGF, rhVEGF‑B

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human PDGF-B Antibody (108128)

Human PDGF-AB ELISA Standard Curve

Human PDGF-AB ELISA Standard Curve

Recombinant Human PDGF‑AB (Catalog # 222-AB) was serially diluted and captured by Mouse Anti-Human PDGF‑B Monoclonal Antibody (Catalog # MAB1739) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human PDGF‑AA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-221-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human PDGF-B Antibody (108128)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human PDGF-B (Catalog # 220-BB)
under non-reducing conditions only

Human PDGF-AB Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human PDGF-AA/AB Biotinylated Antibody (Catalog # BAF221)
  • Standard: Recombinant Human PDGF-AB Protein, CF (Catalog # 222-AB)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 5 using MAB1739 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: PDGF-B

Four PDGF family members, A, B, C and D, have been identified. PDGF-B can exist as a disulfide-linked homodimer (PDGF-BB) or heterodimer (PDGF-AB). PDGF-B binds and activates PDGF R alpha and PDGF R beta.

Long Name

Platelet-derived Growth Factor B

Alternate Names

C-Sis, IBGC5, PDGF-2, PDGF2, PDGFB, SIS, SSV

Entrez Gene IDs

5155 (Human); 24628 (Rat); 100070283 (Equine)

Gene Symbol

PDGFB

UniProt

P01127

Additional PDGF-B Products

Product Documents for Human PDGF-B Antibody (108128)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human PDGF-B Antibody (108128)

For research use only

Related Research Areas

PDGF Family

Citations for Human PDGF-B Antibody (108128)

Customer Reviews for Human PDGF-B Antibody (108128) (1)

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  • Human PDGF-B Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 07/19/2019
    Human PDGF-B Antibody (108128) MAB1739

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