Human PDGF-CC Quantikine ELISA Kit

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Human PDGF-CC ELISA Cell Culture Supernate Standard Curve
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Product Details
Citations (1)
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Human PDGF-CC Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), Platelet-poor EDTA Plasma (50 uL), Platelet-poor Heparin Plasma (50 uL)
24.7 pg/mL
Assay Range
62.5 - 4,000 pg/mL (Cell Culture Supernates, Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma)
Natural and recombinant human PDGF-CC
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
No significant interference observed with available related molecules.

Product Summary

The Quantikine PDGF-CC Immunoassay is a 4.5 hour solid phase ELISA designed to measure human PDGF-CC in cell culture supernates, serum, and platelet-poor plasma. It contains E. coli-expressed recombinant human PDGF-CC and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human PDGF-CC showed linear curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human PDGF-CC.


Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 347 1046 2157 398 1189 2293
Standard Deviation 12.2 26.4 73.6 41.5 102 160
CV% 3.5 2.5 3.4 10.4 8.6 7

Serum, Platelet-poor EDTA Plasma, Platelet-poor Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 479 1407 2777 476 1370 2581
Standard Deviation 21.7 64.1 123 48 104 172
CV% 4.5 4.6 4.4 10.1 7.6 6.7


The recovery of PDGF-CC spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 92 87-97
Platelet-poor EDTA Plasma (n=4) 95 87-104
Platelet-poor Heparin Plasma (n=4) 90 80-99
Serum (n=4) 99 93-108


To assess the linearity of the assay, samples containing and/or spiked with high concentrations of PDGF-CC were diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Scientific Data

Human PDGF-CC ELISA Cell Culture Supernate Standard Curve

Human PDGF-CC ELISA Serum/Platelet-Poor Plasma Standard Curve

Product Datasheets

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Preparation and Storage

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: PDGF-CC

Platelet-derived growth factor C (PDGF-C), also known as SCDGF and follotain, plays an essential role in the regulation of embryonic development, mesenchymal cell proliferation, migration, and survival. It is required for skeleton formation, skin morphogenesis, angiogenesis, blood vessel development, and fibrotic processes. PDGF-C forms disulfide linked dimers which signal through PDGF R alpha homodimeric receptors and PDGF R alpha/beta heterodimeric receptors.

Long Name:
Platelet-derived Growth Factor CC
Entrez Gene IDs:
56034 (Human); 54635 (Mouse)
Alternate Names:
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citation for Human PDGF-CC Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Serum Concentration of Selected Angiogenesis-Related Molecules Differs among Molecular Subtypes, Body Mass Index and Menopausal Status in Breast Cancer Patients
    Authors: D Balalis, D Tsakogiann, E Kalogera, S Kokkali, E Tripodaki, A Ardavanis, D Manatakis, D Dimas, N Koufopoulo, F Fostira, D Korkolis, I Misitzis, N Vassos, C Spiliopoul, D Vlachodimi, G Bletsa, N Arkadopoul
    Oncogene, 2022-07-14;11(14):.
    Species: Human
    Sample Types: Serum


  1. Assay Diluent RD1-63 contains visible precipitate. Is this acceptable to use?

    • A small amount of precipitate is expected and acceptable in Assay Diluent RD1-63. Variability has been observed between vials and diluent lots. It may not fully resolubilize but this will not impact assay performance. It is not recommended to filter the precipitate.

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