Human PDGF-D Antibody

Catalog #: AF1159
5 Citations Datasheet / COA / SDS

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AF1159
AF1159-SP

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Product Details

Citations (5)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human PDGF-D Antibody Summary

Species Reactivity

Human

Specificity

Detects human PDGF-D in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) PDGF-AA, rhPDGF-AB and rhPDGF-BB is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human PDGF-D
Asp253-Arg370
Accession # Q9GZP0

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human PDGF-D

Immunohistochemistry

5-15 µg/mL

Immersion fixed paraffin-embedded sections of human renal cancer tissue

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PDGF-D

PDGF-D, also named spinal cord-derived growth factor B (SCDGF-B) is a member of the PDGF/VEGF family.

Long Name

Platelet-derived Growth Factor D

Entrez Gene IDs

80310 (Human); 71785 (Mouse)

Alternate Names

IEGFMSTP036; Iris-expressed growth factor; PDGFD; PDGF-D; platelet derived growth factor D; SCDGF-BMGC26867; SCDGFBplatelet-derived growth factor D; spinal cord derived growth factor B; Spinal cord-derived growth factor B; spinal cord-derived growth factor-B

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human PDGF-D Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Stromal reprogramming through dual PDGFRalpha/beta blockade boosts the efficacy of anti-PD-1 immunotherapy in fibrotic tumors
Authors: T Akiyama, T Yasuda, T Uchihara, N Yasuda-Yos, BJY Tan, A Yonemura, T Semba, J Yamasaki, Y Komohara, K Ohnishi, F Wei, L Fu, J Zhang, F Kitamura, K Yamashita, K Eto, S Iwagami, H Tsukamoto, T Umemoto, M Masuda, O Nagano, Y Satou, H Saya, P Tan, H Baba, T Ishimoto
Cancer Research, 2023-03-02;0(0):.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
High expression of PDGFR-? in prostate cancer stroma is independently associated with clinical and biochemical prostate cancer recurrence
Authors: Y Nordby, E Richardsen, M Rakaee, N Ness, T Donnem, HR Patel, LT Busund, RM Bremnes, S Andersen
Sci Rep, 2017-02-24;7(0):43378.
Species: Human
Sample Types: Whole Tissue
Applications: IHC
Dynamic regulation of platelet-derived growth factor D (PDGF-D) activity and extracellular spatial distribution by matriptase-mediated proteolysis.
Authors: Huang W, Kim H
J Biol Chem, 2015-02-12;290(14):9162-70.
Species: Human
Sample Types: Cell Lysates
Applications: Immunoprecipitation
Effects of PDGF-C and PDGF-D on monocyte migration and MMP-2 and MMP-9 expression.
Authors: Wagsater D, Zhu C, Bjorck HM, Eriksson P
Atherosclerosis, 2008-05-22;202(2):415-23.
Species: Human
Sample Types: Cell Lysates, Whole Cells, Whole Tissue
Applications: IHC-Fr, Neutralization, Western Blot
Expression of platelet-derived growth factors C and D in the synovial membrane of patients with rheumatoid arthritis and osteoarthritis.
Authors: Pohlers D, Huber R, Ukena B, Kinne RW
Arthritis Rheum., 2006-03-01;54(3):788-94.
Species: Human
Sample Types: Tissue Homogenates, Whole Tissue
Applications: IHC, Western Blot