Human Perforin QuicKit ELISA

Name: Human Perforin QuicKit ELISA
Brand: R&D Systems
SKU: QK8011
Price: 720 USD
Availability: InStock

R&D Systems | Catalog # QK8011

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Product Summary
Product Specifications
Precision
Recovery
Linearity
Scientific Data
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Assay Length

80 minutes

Sample Type & Volume Required Per Well

Cell Culture Supernates (50 µL), Cell Lysates (50 µL), Serum (13 µL), EDTA Plasma (13 µL), Heparin Plasma (13 µL)

Sensitivity

14 pg/mL

Assay Range

62.5-4000 pg/mL (Cell Culture Supernates, Cell Lysates, Serum, EDTA Plasma, Heparin Plasma)

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Product Summary for Human Perforin QuicKit ELISA

The Quantikine QuicKit Human Perforin Immunoassay is a one step, 80-minute solid phase ELISA designed to measure human Perforin levels in cell culture supernates, serum, plasma, and cell lysates. It contains recombinant human Perforin and antibodies raised against the recombinant protein. Results obtained for naturally occurring human Perforin showed linear curves that were parallel to the standard curves obtained using the recombinant QuicKit standards. These results indicate that this kit can be used to determine relative mass values for natural Perforin.

Product Specifications

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human Perforin.

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

No significant interference observed with available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Two samples of known concentration were tested twenty times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Two samples of known concentration were tested in ten separate assays to assess inter-assay precision. Assays were performed by at least five technicians.

Cell Culture Supernates, Cell Lysates, EDTA Plasma, Heparin Plasma, Serum

	Intra-Assay Precision		Inter-Assay Precision
Sample	1	2	1	2
n	20	20	10	10
Mean (pg/mL)	647	2885	477	2689
Standard Deviation	36.6	77.7	33.0	95.4
CV%	5.7	2.7	6.9	3.5

Recovery for Human Perforin QuicKit ELISA

The recovery of human Perforin spiked to three different levels in samples throughout the range of the assay in various matrices was evaluated.

Sample Type	Average % Recovery	Range %
Cell Culture Media (n=4)	103	82-132
EDTA Plasma (n=2)	100	81-122
Heparin Plasma (n=2)	103	87-118
Lysis Buffer (n=1)	118	109-127
Serum (n=2)	97	73-117

Linearity

To assess the linearity of the assay, samples containing high concentrations of human Perforin were diluted with calibrator diluent to produce samples with values within the dynamic range of the assay. Samples were diluted prior to assay.

Scientific Data Images for Human Perforin QuicKit ELISA

Human Perforin Quantikine QuicKit ELISA Standard Curve

Human Perforin QuicKit Serum Recovery

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.