Human Phospho-53BP1 (S25) Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Human Phospho-53BP1 (S25) by Western Blot. Western blot shows lysates of K562 human chronic myelogenous leukemia cell line untreated (-) or exposed (+) to 50 J/m2UV-C and allowed 1 hour recovery time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-53BP1 (S25) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3405) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-53BP1 (S25) was detected at approximately 450 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
The p53-binding protein 1 (53BP1) localizes to sites of damaged DNA and is phosphorylated by the ATM checkpoint kinase. 53BP1 is required for phosphorylation of other downstream ATM substrates, such as p53 and SMC-1, and is itself a substrate for the ATM kinase. ATM phosphorylates 53BP1 at S25, and this phosphorylation aids in 53BP1 nucleating a DNA damage response protein complex.
Citation for Human Phospho-53BP1 (S25) Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
53BP1 promotes ATM activity through direct interactions with the MRN complex.
Authors: Lee JH, Goodarzi AA, Jeggo PA, Paull TT
EMBO J., 2009-12-10;29(3):574-85.
Sample Types: Cell Lysates
Applications: Western Blot
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