Human phospho-Chk1 (S345) Antibody

R&D Systems | Catalog # AF2475

R&D Systems

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Chk1 Primary Antibodies »

Product Specifications

Immunogen

Phosphopeptide containing human Chk1 S345 site

Specificity

Detects human, mouse, and rat Chk1 when phosphorylated at S345. This antibody does not recognize Chk1 when unphosphorylated at S345.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human phospho-Chk1 (S345) Antibody

Detection of Human Phospho-Chk1 (S345) antibody by Western Blot.

Detection of Human Phospho-Chk1 (S345) by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line exposed to 50 J/m2UV-C with a 2 hour recovery. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human Phospho-Chk1 (S345) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2475) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk1 (S345) was detected at approximately 55-60 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human phospho-Chk1 (S345) Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line exposed to UV-C

Reviewed Applications

Read 1 review rated 5 using AF2475 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chk1

The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic or replicative stress, Chk1 is phosphorylated by the ATM or ATM-related kinase (ATR) at S345. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.

Long Name

Checkpoint Kinase 1

Alternate Names

CHEK1

Entrez Gene IDs

1111 (Human); 12649 (Mouse); 140583 (Rat)

Gene Symbol

CHEK1

Additional Chk1 Products

Product Documents for Human phospho-Chk1 (S345) Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human phospho-Chk1 (S345) Antibody

For research use only

Customer Reviews for Human phospho-Chk1 (S345) Antibody (1)

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  • Human Phospho-Chk1 (S345) Antibody
    Name: Isabelle Cristine da Costa
    Application: Western Blot
    Sample Tested: U2OS human osteosarcoma cell line
    Species: Human
    Verified Customer | Posted 09/23/2021
    Excellent antibody, it works very well for western blotting.
    Human phospho-Chk1 (S345) Antibody AF2475

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